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dc.contributor.authorKuck, L
dc.contributor.authorPeart, JN
dc.contributor.authorSimmonds, MJ
dc.date.accessioned2020-08-03T03:20:19Z
dc.date.available2020-08-03T03:20:19Z
dc.date.issued2020
dc.identifier.issn0167-4889
dc.identifier.doi10.1016/j.bbamcr.2020.118802
dc.identifier.urihttp://hdl.handle.net/10072/396065
dc.description.abstractRed blood cells (RBC) are constantly exposed to varying mechanical forces while traversing the cardiovascular system. Upon exposure to mechanical stimuli (e.g., shear stress), calcium enters the cell and prompts potassium-efflux. Efflux of potassium is accompanied by a loss of intracellular fluid; thus, the volume of RBC decreases proportionately (i.e., 'Gárdos effect'). The mechanical properties of the cell are subsequently impacted due to complex interactions between cytosolic viscosity (dependent on cell hydration), the surface-area-to-volume ratio, and other molecular processes. The dynamic effects of calcium on RBC mechanics are yet to be elucidated, although accumulating evidence suggests a vital role. The present study thus examined the effects of calcium on contemporary biomechanical properties of RBC in conjunction with high-precision geometrical analyses with exposure to shear. Mechanical stimulation of RBC was performed using a co-axial Couette shearing system to deform the cell membrane; intracellular signaling events were observed via fluorescent imaging. Calcium was introduced into RBC using ionophore A23187. Increased intracellular calcium significantly impaired RBC deformability; these impairments were mediated by a calcium-induced reduction of cell volume through the Gárdos channel. Extracellular calcium in the absence of the ionophore only had an effect under shear, not at stasis. Under low shear, the presence of extracellular calcium induced progressive lysis of a sub-population of RBC; all remaining RBC exhibited exceptional capacity to deform, implying preferential removal of potentially aged cells. Collectively, we provide evidence of the mechanism by which calcium acutely regulates RBC mechanical properties.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom118802
dc.relation.ispartofjournalBiochimica et Biophysica Acta (BBA) - Molecular Cell Research
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3207
dc.subject.keywordsCalcium signaling
dc.subject.keywordsCell deformability
dc.subject.keywordsMechanobiology
dc.subject.keywordsMechanotransduction
dc.subject.keywordsRed blood cell
dc.titleCalcium dynamically alters erythrocyte mechanical response to shear
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationKuck, L; Peart, JN; Simmonds, MJ, Calcium dynamically alters erythrocyte mechanical response to shear, Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2020, pp. 118802
dcterms.dateAccepted2020-07-20
dc.date.updated2020-08-03T01:30:22Z
gro.hasfulltextNo Full Text
gro.griffith.authorPeart, Jason N.
gro.griffith.authorSimmonds, Michael J.
gro.griffith.authorKuck, Lennart L.


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