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dc.contributor.authorSparks, David S
dc.contributor.authorSaifzadeh, Siamak
dc.contributor.authorSavi, Flavia Medeiros
dc.contributor.authorDlaska, Constantin E
dc.contributor.authorBerner, Arne
dc.contributor.authorHenkel, Jan
dc.contributor.authorReichert, Johannes C
dc.contributor.authorWullschleger, Martin
dc.contributor.authorRen, Jiongyu
dc.contributor.authorCipitria, Amaia
dc.contributor.authorMcGovern, Jacqui A
dc.contributor.authorSteck, Roland
dc.contributor.authorWagels, Michael
dc.contributor.authorWoodruff, Maria Ann
dc.contributor.authorSchuetz, Michael A
dc.contributor.authorHutmacher, Dietmar W
dc.date.accessioned2020-08-12T04:43:19Z
dc.date.available2020-08-12T04:43:19Z
dc.date.issued2020
dc.identifier.issn1754-2189
dc.identifier.doi10.1038/s41596-019-0271-2
dc.identifier.urihttp://hdl.handle.net/10072/396456
dc.description.abstractCritical-size bone defects, which require large-volume tissue reconstruction, remain a clinical challenge. Bone engineering has the potential to provide new treatment concepts, yet clinical translation requires anatomically and physiologically relevant preclinical models. The ovine critical-size long-bone defect model has been validated in numerous studies as a preclinical tool for evaluating both conventional and novel bone-engineering concepts. With sufficient training and experience in large-animal studies, it is a technically feasible procedure with a high level of reproducibility when appropriate preoperative and postoperative management protocols are followed. The model can be established by following a procedure that includes the following stages: (i) preoperative planning and preparation, (ii) the surgical approach, (iii) postoperative management, and (iv) postmortem analysis. Using this model, full results for peer-reviewed publication can be attained within 2 years. In this protocol, we comprehensively describe how to establish proficiency using the preclinical model for the evaluation of a range of bone defect reconstruction options.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherNature Publishing Group
dc.publisher.placeUnited Kingdom
dc.relation.ispartofpagefrom877
dc.relation.ispartofpageto924
dc.relation.ispartofissue3
dc.relation.ispartofjournalNature Protocols
dc.relation.ispartofvolume15
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchChemical Sciences
dc.subject.fieldofresearchcode11
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode03
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsBiochemical Research Methods
dc.subject.keywordsBiochemistry & Molecular Biology
dc.subject.keywordsOVINE MODEL
dc.titleA preclinical large-animal model for the assessment of critical-size load-bearing bone defect reconstruction
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationSparks, DS; Saifzadeh, S; Savi, FM; Dlaska, CE; Berner, A; Henkel, J; Reichert, JC; Wullschleger, M; Ren, J; Cipitria, A; McGovern, JA; Steck, R; Wagels, M; Woodruff, MA; Schuetz, MA; Hutmacher, DW, A preclinical large-animal model for the assessment of critical-size load-bearing bone defect reconstruction, Nature Protocols, 2020, 15 (3), pp. 877-924
dcterms.dateAccepted2019-11-11
dc.date.updated2020-08-12T04:41:17Z
gro.hasfulltextNo Full Text
gro.griffith.authorWullschleger, Martin
gro.griffith.authorHutmacher, Dietmar W.


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