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dc.contributor.authorSparks, David S
dc.contributor.authorSaifzadeh, Siamak
dc.contributor.authorSavi, Flavia Medeiros
dc.contributor.authorDlaska, Constantin E
dc.contributor.authorBerner, Arne
dc.contributor.authorHenkel, Jan
dc.contributor.authorReichert, Johannes C
dc.contributor.authorWullschleger, Martin
dc.contributor.authorRen, Jiongyu
dc.contributor.authorCipitria, Amaia
dc.contributor.authorMcGovern, Jacqui A
dc.contributor.authorSteck, Roland
dc.contributor.authorWagels, Michael
dc.contributor.authorWoodruff, Maria Ann
dc.contributor.authorSchuetz, Michael A
dc.contributor.authorHutmacher, Dietmar W
dc.description.abstractCritical-size bone defects, which require large-volume tissue reconstruction, remain a clinical challenge. Bone engineering has the potential to provide new treatment concepts, yet clinical translation requires anatomically and physiologically relevant preclinical models. The ovine critical-size long-bone defect model has been validated in numerous studies as a preclinical tool for evaluating both conventional and novel bone-engineering concepts. With sufficient training and experience in large-animal studies, it is a technically feasible procedure with a high level of reproducibility when appropriate preoperative and postoperative management protocols are followed. The model can be established by following a procedure that includes the following stages: (i) preoperative planning and preparation, (ii) the surgical approach, (iii) postoperative management, and (iv) postmortem analysis. Using this model, full results for peer-reviewed publication can be attained within 2 years. In this protocol, we comprehensively describe how to establish proficiency using the preclinical model for the evaluation of a range of bone defect reconstruction options.
dc.publisherNature Publishing Group
dc.publisher.placeUnited Kingdom
dc.relation.ispartofjournalNature Protocols
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchChemical Sciences
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsBiochemical Research Methods
dc.subject.keywordsBiochemistry & Molecular Biology
dc.subject.keywordsOVINE MODEL
dc.titleA preclinical large-animal model for the assessment of critical-size load-bearing bone defect reconstruction
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationSparks, DS; Saifzadeh, S; Savi, FM; Dlaska, CE; Berner, A; Henkel, J; Reichert, JC; Wullschleger, M; Ren, J; Cipitria, A; McGovern, JA; Steck, R; Wagels, M; Woodruff, MA; Schuetz, MA; Hutmacher, DW, A preclinical large-animal model for the assessment of critical-size load-bearing bone defect reconstruction, Nature Protocols, 2020, 15 (3), pp. 877-924
gro.hasfulltextNo Full Text
gro.griffith.authorWullschleger, Martin
gro.griffith.authorHutmacher, Dietmar W.

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