Show simple item record

dc.contributor.authorMcNamee, Antony P
dc.contributor.authorTansley, Geoff D
dc.contributor.authorSimmonds, Michael J
dc.date.accessioned2020-08-17T03:20:23Z
dc.date.available2020-08-17T03:20:23Z
dc.date.issued2020
dc.identifier.issn1549-8719
dc.identifier.doi10.1111/micc.12652
dc.identifier.urihttp://hdl.handle.net/10072/396492
dc.description.abstractBlood exposure to supraphysiological shear stress within mechanical circulatory support is suspected of reducing red blood cell (RBC) deformability and being primal in the pathogenesis of several secondary complications. No prior works have explored RBC dynamics with the resolution required to determine shear elastic modulus, and/or cell capillary velocity, following exposure to mechanical stresses. Healthy RBCs were exposed to 0,5,50, and 100Pa in a Couette shearing system. For comparison, blood was also exposed to heat treatment - a method that predictably increases RBC rigidity. Shear modulus assessment required aspiration of single RBCs through narrow micropipettes at known suction force. Cell transit velocities were measured within micro-channels in regions of fully-developed flow. Supraphysiological shear stress increased the elastic shear modulus by 39% and 69% following exposure to 50 and 100Pa, respectively. Cell transit velocity, however, did not change following shear, with concurrent decreases in cell volume likely nullifying increased shear modulus-friction interactions. Differences observed were consistent with our internal control (heat treatment), supporting that cell mechanics are significantly impaired following supraphysiological-sublethal shear exposure. Given mechanical circulatory support operates at shear stresses consistent with the present study, it is plausible that these devices induce fundamental impairment to the material properties of RBCs.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofjournalMicrocirculation
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchcode32
dc.subject.fieldofresearchcode31
dc.subject.keywordsRBC deformability
dc.subject.keywordshaemorheology
dc.subject.keywordsmechanobiology
dc.subject.keywordsmicropipette aspiration
dc.subject.keywordsrheology
dc.titleSublethal mechanical shear stress increases the elastic shear modulus of red blood cells but does not change capillary transit velocity.
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationMcNamee, AP; Tansley, GD; Simmonds, MJ, Sublethal mechanical shear stress increases the elastic shear modulus of red blood cells but does not change capillary transit velocity., Microcirculation, 2020
dc.date.updated2020-08-15T21:07:40Z
dc.description.versionAccepted Manuscript (AM)
gro.description.notepublicThis publication has been entered in Griffith Research Online as an advanced online version.
gro.rights.copyright© 2020 Wiley-Liss, Inc. This is the peer reviewed version of the following article: Sublethal mechanical shear stress increases the elastic shear modulus of red blood cells but does not change capillary transit velocity., Microcirculation, 2020, which has been published in final form at https://doi.org/10.1111/micc.12652. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)
gro.hasfulltextFull Text
gro.griffith.authorSimmonds, Michael J.
gro.griffith.authorMcNamee, Antony
gro.griffith.authorTansley, Geoff


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record