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dc.contributor.authorGholami, Mahnaz D
dc.contributor.authorSonar, Prashant
dc.contributor.authorAyoko, Godwin A
dc.contributor.authorIzake, Emad L
dc.date.accessioned2020-08-31T00:50:35Z
dc.date.available2020-08-31T00:50:35Z
dc.date.issued2020
dc.identifier.issn0925-4005
dc.identifier.doi10.1016/j.snb.2020.127867
dc.identifier.urihttp://hdl.handle.net/10072/396900
dc.description.abstractTumor necrosis factor alpha (TNF-α) is a cytokine that plays a critical role in medical conditions such cardiovascular diseases, rheumatoid arthritis, inflammatory bowel disease, Alzheimer’s and cancer. Herein, we present a new method for the determination of TNF-α by surface enhanced Raman spectroscopy (SERS). A new benzothiazole azo dye (BAN) was used as a Raman probe to detect the cytokine after its selective extraction from blood plasma using a target-specific antibody-functionalised extractor chip. The disulfide bond structure of the extracted TNF-α was reduced to generate free sulfhydryl (SH), terminal groups that adsorb preferentially onto a BAN-functionalised SERS substrate and displace the BAN Raman reporter on the substrate surface. This causes the SERS spectrum of BAN to quench proportionally with the cytokine concentration. Using this SERS quenching sensor, TNF-α was quantified down to 1 × 10−14 M (173 pg/L). The quantification of the cytokine by the SERS quenching method was cross-validated against enzyme-linked immunosorbent assay (ELISA) and the percent agreement between the two measurements was found to be 93.39 %. Since many proteins and peptides have disulfide bonds in their molecular structures, the new SERS quenching method can be extended for their ultrasensitive quantification after selective extraction from biological fluids.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom127867
dc.relation.ispartofjournalSensors and Actuators B: Chemical
dc.relation.ispartofvolume310
dc.subject.fieldofresearchAtomic, molecular and optical physics
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchMaterials engineering
dc.subject.fieldofresearchcode5102
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode4016
dc.subject.keywordsScience & Technology
dc.subject.keywordsPhysical Sciences
dc.subject.keywordsElectrochemistry
dc.titleA highly sensitive SERS quenching nanosensor for the determination of tumor necrosis factor alpha in blood
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationGholami, MD; Sonar, P; Ayoko, GA; Izake, EL, A highly sensitive SERS quenching nanosensor for the determination of tumor necrosis factor alpha in blood, Sensors and Actuators B: Chemical, 2020, 310, pp. 127867
dc.date.updated2020-08-31T00:48:58Z
gro.hasfulltextNo Full Text
gro.griffith.authorSonar, Prashant


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