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dc.contributor.authorLee, KTW
dc.contributor.authorIslam, F
dc.contributor.authorVider, J
dc.contributor.authorMartin, J
dc.contributor.authorChruścik, A
dc.contributor.authorLu, CT
dc.contributor.authorGopalan, V
dc.contributor.authorKing-Yin Lam, A
dc.date.accessioned2020-09-07T23:31:56Z
dc.date.available2020-09-07T23:31:56Z
dc.date.issued2020
dc.identifier.issn1538-4047
dc.identifier.doi10.1080/15384047.2020.1810535
dc.identifier.urihttp://hdl.handle.net/10072/397125
dc.description.abstractThis study aims to investigate the overexpression-induced properties of tumor suppressor FAM134B (family with sequence similarity 134, member B) in colon cancer, examine the potential gene regulators of FAM134B expression and its impact on mitochondrial function. FAM134B was overexpressed in colon cancer and non-neoplastic colonic epithelial cells. Various cell-based assays including apoptosis, cell cycle, cell proliferation, clonogenic, extracellular flux and wound healing assays were performed. Western blot analysis was used to confirm and identify potential interacting partners of FAM134B in vitro. Immunohistochemistry and qPCR were employed to determine the expressions of MIF and FAM134B, respectively, on 63 patients with colorectal carcinoma. Results showed that FAM134B is involved in the cell cycle and mitochondrial function of colon cancer. Overexpression of FAM134B was coupled with increased expression levels of APC, p53, and MIF. Increased expression of both APC and p53 further validates the potential role of tumor suppressor FAM134B in regulating cancer progression through the WNT/β-catenin signaling pathway. In approximately 70% of the patients with colorectal cancer, FAM134B downregulation was correlated with MIF protein overexpression while the remaining 30% showed concurrent expression of FAM134B and MIF (P = .045). High expression of MIF coupled with low expression of FAM134B is associated with microsatellite instability status in colorectal carcinomas (P = .049). FAM134B may exert its tumor suppressive function through affecting cell cycle, mitochondrial function via potentially interacting with MIF and p53.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherInforma UK Limited
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto9
dc.relation.ispartofjournalCancer Biology and Therapy
dc.subject.fieldofresearchOncology and carcinogenesis
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchcode3211
dc.subject.fieldofresearchcode3101
dc.subject.keywordsFAM134B
dc.subject.keywordsMIF
dc.subject.keywordscancer
dc.subject.keywordscolon
dc.subject.keywordscolorectal
dc.titleOverexpression of family with sequence similarity 134, member B (FAM134B) in colon cancers and its tumor suppressive properties in vitro
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationLee, KTW; Islam, F; Vider, J; Martin, J; Chruścik, A; Lu, CT; Gopalan, V; King-Yin Lam, A, Overexpression of family with sequence similarity 134, member B (FAM134B) in colon cancers and its tumor suppressive properties in vitro, Cancer Biology and Therapy, 2020, pp. 1-9
dc.date.updated2020-09-07T00:43:55Z
dc.description.versionAccepted Manuscript (AM)
gro.description.notepublicThis publication has been entered into Griffith Research Online as an Advanced Online Version.
gro.rights.copyrightThis is an Author's Accepted Manuscript of an article published in Cancer Biology & Therapy, Latest Articles, 28 Aug 2020, copyright Taylor & Francis, available online at: https://doi.org/10.1080/15384047.2020.1810535
gro.hasfulltextFull Text
gro.griffith.authorGopalan, Vinod
gro.griffith.authorLam, Alfred K.
gro.griffith.authorIslam, Farhad


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