Serum levels of leptin, osteopontin, and sclerostin in patients with and without knee osteoarthritis

Abstract

Objective: To investigate the relationship between leptin, osteopontin (OPN), sclerostin (SOST) and severity of knee osteoarthritis (KOA). Methods: The study included 148 consecutive patients with knee OA and 101 non-KOA subjects enrolled in this cross-sectional study. All patients fulfilled the American College of Rheumatology criteria for primary knee OA. Severity of the disease was assessed using plain radiography of the affected knee, according to the Kellgren and Lawrence classification. Fasting blood samples were obtained from all patients and controls; the serum samples were kept at − 80 °C before assessment of leptin, OPN, and SOST using a multiplex particle-based flow cytometric assay. Results: KOA patients group compared with the control group, serum leptin (KOA, 26581.7 ± 2011.5 pg/ml, vs control,6936.4 ± 702.2 pg/ml),OPN (KOA, 4908.3 ± 769.4 pg/ml, vs control, 2182.5 ± 217.8 pg/ml), and SOST (KOA, 2481.9 ± 543.5 pg/ml, vs control, 1288.9 ± 267.7 pg/ml) in the KOA group were higher than control group; there were also differences in three bone metabolic factors between male and female in the KOA group; meanwhile, there was correlation between each factor and the incidence of KOA. Conclusion: Our study of 249 serum samples was conducted. Serum leptin, OPN, and SOST were significantly increased in KOA patients, and there was an internal correlation; these findings could, at best, contribute to the identification of novel targets for medical interventions.Key Points• The aim of this study was to assess the relationships of radiographic knee OA with altered serum levels of leptin, OPN, and SOST. Our study of 249 serum samples was conducted. Serum leptin, OPN, and SOST were significantly increased in KOA patients compared with control group. There were gender differences in the concentration of three serum bone turnover factors in KOA group and control group. Serum SOST concentration increased with Kellgren-Lawrence (K-L) grading. We found that serum leptin, OPN, and SOST were significantly increased in KOA patients, and there was an internal correlation. Leptin had a remarkable diagnostic value in the incidence of KOA.