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dc.contributor.authorSuzuki, Shuko
dc.contributor.authorShadforth, Audra MA
dc.contributor.authorMcLenachan, Samuel
dc.contributor.authorZhang, Dan
dc.contributor.authorChen, Shang-Chih
dc.contributor.authorWalshe, Jennifer
dc.contributor.authorLidgerwood, Grace E
dc.contributor.authorPebay, Alice
dc.contributor.authorChirila, Traian
dc.contributor.authorChen, Fred K
dc.contributor.authorHarkin, Damien G
dc.date.accessioned2020-09-10T00:33:47Z
dc.date.available2020-09-10T00:33:47Z
dc.date.issued2019
dc.identifier.issn0928-4931
dc.identifier.doi10.1016/j.msec.2019.110131
dc.identifier.urihttp://hdl.handle.net/10072/397282
dc.description.abstractSilk fibroin membrane displays potential for ocular tissue reconstruction as demonstrated by its ability to support a functioning retinal pigment epithelium (RPE) in vitro. Nevertheless, translation of these findings to the clinic will require the use of membranes that can be readily handled and implanted into diseased retinas, with minimal impact on the surrounding healthy tissue. To this end, we optimized the physical properties of fibroin membranes to enable surgical handling during implantation into the retina, without compromising biocompatibility or permeability. Our central hypothesis is that optimal strength and permeability can be achieved by combining the porogenic properties of poly(ethylene glycol) (PEG) with the crosslinking properties of horseradish peroxidase (HRP). Our study reveals that PEG used in conjunction with HRP enables the production of fibroin membranes with superior handling properties to conventional fibroin membranes. More specifically, the modified membranes could be more easily implanted into the retinas of rats and displayed good evidence of biocompatibility. Moreover, the modified membranes retained the ability to support construction of functional RPE derived from pluripotent stem cells. These findings pave the way for preclinical studies of RPE-implantation using the optimized fibroin membranes.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofjournalMaterials Science and Engineering C
dc.relation.ispartofvolume105
dc.subject.fieldofresearchBiomedical engineering
dc.subject.fieldofresearchMaterials engineering
dc.subject.fieldofresearchcode4003
dc.subject.fieldofresearchcode4016
dc.subject.keywordsScience & Technology
dc.subject.keywordsTechnology
dc.subject.keywordsMaterials Science, Biomaterials
dc.subject.keywordsMaterials Science
dc.subject.keywordsSilk fibroin
dc.titleOptimization of silk fibroin membranes for retinal implantation
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationSuzuki, S; Shadforth, AMA; McLenachan, S; Zhang, D; Chen, S-C; Walshe, J; Lidgerwood, GE; Pebay, A; Chirila, T; Chen, FK; Harkin, DG, Optimization of silk fibroin membranes for retinal implantation, Materials Science and Engineering C, 2019, 105
dcterms.dateAccepted2019-08-23
dc.date.updated2020-09-10T00:32:42Z
gro.hasfulltextNo Full Text
gro.griffith.authorShadforth, Audra


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