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dc.contributor.authorBuddawong, Tiranan
dc.contributor.authorAsuvapongpatana, Somluk
dc.contributor.authorSenapin, Saengchan
dc.contributor.authorMcDougall, Carmel
dc.contributor.authorWeerachatyanukul, Wattana
dc.date.accessioned2020-09-15T06:26:58Z
dc.date.available2020-09-15T06:26:58Z
dc.date.issued2020
dc.identifier.issn2167-8359
dc.identifier.doi10.7717/peerj.8868
dc.identifier.urihttp://hdl.handle.net/10072/397529
dc.description.abstractCalcineurin (CN) is known to be involved in many biological processes, particularly, the immune response mechanism in many invertebrates. In this study, we characterized both HcCNA and HcCNB genes in Haliotis diversicolor, documented their expression in many tissues, and discerned their function as immune responsive genes against Vibrio parahaemolyticus infection. Similar to other mollusk CNs, the HcCNA gene lacked a proline-rich domain and comprised only one isoform of its catalytic unit, in contrast to CNs found in mammals. HcCNB was highly conserved in both sequence and domain architecture. Quantitative PCR and in situ hybridization revealed that the genes were broadly expressed and were not restricted to tissues traditionally associated with immune function. Upon infection of H. diversicolor with V. parahaemolyticus (a bacteria that causes serious disease in crustaceans and mollusks), both HcCNA and HcCNB genes were highly up-regulated at the early phase of bacterial infection. HcCNB was expressed significantly higher than HcCNA in response to bacterial challenge, suggesting its independent or more rapid response to bacterial infection. Together, the two CN genes are unique in their gene structure (particular HcCNA) and distribution in mollusk species and likely function as immune responsive genes along with many other genes that are enhanced in the early phase of V. parahaemolyticus infection in abalone.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherPeerJ, Ltd.
dc.relation.ispartofissue4
dc.relation.ispartofjournalPeerJ
dc.relation.ispartofvolume8
dc.subject.fieldofresearchAquaculture
dc.subject.fieldofresearchAnimal immunology
dc.subject.fieldofresearchMolecular evolution
dc.subject.fieldofresearchcode300501
dc.subject.fieldofresearchcode310905
dc.subject.fieldofresearchcode310510
dc.subject.keywordsScience & Technology
dc.subject.keywordsMultidisciplinary Sciences
dc.subject.keywordsScience & Technology - Other Topics
dc.subject.keywordsCalcineurin
dc.subject.keywordsCNA
dc.titleCharacterization of calcineurin A and B genes in the abalone, Haliotis diversicolor, and their immune response role during bacterial infection
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationBuddawong, T; Asuvapongpatana, S; Senapin, S; McDougall, C; Weerachatyanukul, W, Characterization of calcineurin A and B genes in the abalone, Haliotis diversicolor, and their immune response role during bacterial infection, PeerJ, 2020, 8 (4)
dcterms.dateAccepted2020-03-09
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2020-09-15T06:23:15Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2020 Buddawong et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorMcDougall, Carmel


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