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  • Assigning a role for chemosensory signal transduction in Campylobacter jejuni biofilms using a combined omics approach

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    Tram422192-Published.pdf (1.807Mb)
    Author(s)
    Tram, Greg
    Klare, William P
    Cain, Joel A
    Mourad, Basem
    Cordwell, Stuart J
    Day, Christopher J
    Korolik, Victoria
    Griffith University Author(s)
    Korolik, Victoria
    Day, Christopher J.
    Mourad, Basem
    Tram, Greg C.
    Year published
    2020
    Metadata
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    Abstract
    Biofilms of the gastroenteric pathogen C. jejuni may serve an important role in the transmission of infection from reservoirs of infection to humans. Herein, we undertook a combinatorial approach examining differential gene expression and protein abundance during biofilm formation in C. jejuni. Biofilms induced a substantial rearrangement of the C. jejuni transcriptome and proteome, with ~600 genes differentially expressed when compared to planktonic cells. Genes and proteins induced in biofilms were involved in iron metabolism and acquisition, cell division, glycan production and attachment, while those repressed were ...
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    Biofilms of the gastroenteric pathogen C. jejuni may serve an important role in the transmission of infection from reservoirs of infection to humans. Herein, we undertook a combinatorial approach examining differential gene expression and protein abundance during biofilm formation in C. jejuni. Biofilms induced a substantial rearrangement of the C. jejuni transcriptome and proteome, with ~600 genes differentially expressed when compared to planktonic cells. Genes and proteins induced in biofilms were involved in iron metabolism and acquisition, cell division, glycan production and attachment, while those repressed were associated with metabolism, amino acid usage, and large tracts of the chemotaxis pathway. We further examined the role of chemotaxis in C. jejuni biofilm formation by examining isogenic strains with deletions of the cheV and cheW signal transduction genes. Both ∆cheV and ∆cheW exhibited a significant decrease in directed motility when compared to wild-type C. jejuni as well as demonstrating an increase in autoagglutination ability and biofilm formation. A subtle difference was also observed between the phenotypes of ∆cheV and ∆cheW mutants, both in motility and biofilm formation. This suggests roles for CheV and CheW and may present signal transduction as a potential method for modulating C. jejuni biofilm formation.
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    Journal Title
    Scientific Reports
    Volume
    10
    Issue
    1
    DOI
    https://doi.org/10.1038/s41598-020-63569-5
    Copyright Statement
    © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
    Subject
    Science & Technology
    Multidisciplinary Sciences
    Science & Technology - Other Topics
    BACILLUS-SUBTILIS
    CHEMOTAXIS
    Publication URI
    http://hdl.handle.net/10072/397533
    Collection
    • Journal articles

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