Show simple item record

dc.contributor.authorRussell, Jake S
dc.contributor.authorGriffith, Tia A
dc.contributor.authorNaghipour, Saba
dc.contributor.authorVider, Jelena
dc.contributor.authorDu Toit, Eugene F
dc.contributor.authorPatel, Hemal H
dc.contributor.authorPeart, Jason N
dc.contributor.authorHeadrick, John P
dc.date.accessioned2020-09-16T01:25:58Z
dc.date.available2020-09-16T01:25:58Z
dc.date.issued2020
dc.identifier.issn2072-6643
dc.identifier.doi10.3390/nu12092679
dc.identifier.urihttp://hdl.handle.net/10072/397551
dc.description.abstractWhether dietary omega-3 (n-3) polyunsaturated fatty acid (PUFA) confers cardiac benefit in cardiometabolic disorders is unclear. We test whether dietary -linolenic acid (ALA) enhances myocardial resistance to ischemia-reperfusion (I-R) and responses to ischemic preconditioning (IPC) in type 2 diabetes (T2D); and involvement of conventional PUFA-dependent mechanisms (caveolins/cavins, kinase signaling, mitochondrial function, and inflammation). Eight-week male C57Bl/6 mice received streptozotocin (75 mg/kg) and 21 weeks high-fat/high-carbohydrate feeding. Half received ALA over six weeks. Responses to I-R/IPC were assessed in perfused hearts. Localization and expression of caveolins/cavins, protein kinase B (AKT), and glycogen synthase kinase-3 β (GSK3β); mitochondrial function; and inflammatory mediators were assessed. ALA reduced circulating leptin, without affecting body weight, glycemic dysfunction, or cholesterol. While I-R tolerance was unaltered, paradoxical injury with IPC was reversed to cardioprotection with ALA. However, post-ischemic apoptosis (nucleosome content) appeared unchanged. Benefit was not associated with shifts in localization or expression of caveolins/cavins, p-AKT, p-GSK3β, or mitochondrial function. Despite mixed inflammatory mediator changes, tumor necrosis factor-a (TNF-a) was markedly reduced. Data collectively reveal a novel impact of ALA on cardioprotective dysfunction in T2D mice, unrelated to caveolins/cavins, mitochondrial, or stress kinase modulation. Although evidence suggests inflammatory involvement, the basis of this "un-conventional" protection remains to be identified.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherMDPI AG
dc.relation.ispartofpagefrom2679
dc.relation.ispartofissue9
dc.relation.ispartofjournalNutrients
dc.relation.ispartofvolume12
dc.subject.fieldofresearchFood Sciences
dc.subject.fieldofresearchNutrition and Dietetics
dc.subject.fieldofresearchcode0908
dc.subject.fieldofresearchcode1111
dc.subject.keywords-linolenic acid
dc.subject.keywordscardioprotection
dc.subject.keywordscaveolae
dc.subject.keywordscaveolins
dc.subject.keywordscavins
dc.titleDietary α-Linolenic Acid Counters Cardioprotective Dysfunction in Diabetic Mice: Unconventional PUFA Protection
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationRussell, JS; Griffith, TA; Naghipour, S; Vider, J; Du Toit, EF; Patel, HH; Peart, JN; Headrick, JP, Dietary α-Linolenic Acid Counters Cardioprotective Dysfunction in Diabetic Mice: Unconventional PUFA Protection., Nutrients, 2020, 12 (9), pp. 2679
dcterms.dateAccepted2020-08-26
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2020-09-16T00:01:05Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorRussell, Jake S.
gro.griffith.authorHeadrick, John P.
gro.griffith.authorDu Toit, Eugene
gro.griffith.authorPeart, Jason N.
gro.griffith.authorNaghipour, Saba
gro.griffith.authorGriffith, Tia A.
gro.griffith.authorVider, Jelena


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record