Show simple item record

dc.contributor.authorO'Dea, Christian
dc.contributor.authorZhang, Qian
dc.contributor.authorStaley, Christopher
dc.contributor.authorMasters, Nicole
dc.contributor.authorKuballa, Anna
dc.contributor.authorFisher, Paul
dc.contributor.authorVeal, Cameron
dc.contributor.authorStratton, Helen
dc.contributor.authorSadowsky, Michael J
dc.contributor.authorAhmed, Warish
dc.contributor.authorKatouli, Mohammad
dc.date.accessioned2020-09-18T01:13:47Z
dc.date.available2020-09-18T01:13:47Z
dc.date.issued2019
dc.identifier.issn0043-1354
dc.identifier.doi10.1016/j.watres.2019.114967
dc.identifier.urihttp://hdl.handle.net/10072/397637
dc.description.abstractCharacterization of microbial communities using high-throughput amplicon sequencing is an emerging approach for microbial source tracking of fecal pollution. This study used SourceTracker software to examine temporal and geographical variability of fecal bacterial community profiles to identify pollutant sources in three freshwater catchments in sub-tropical Australia. Fecal bacterial communities from 10 animal species, humans, and composite wastewater samples from six sewage treatment plants were characterized and compared to freshwater samples using Illumina amplicon sequencing of the V5–V6 regions of the 16S rRNA gene. Source contributions were calculated in SourceTracker using new fecal taxon libraries as well as previously generated libraries to determine the effects of geographic and temporal variability on source assignments. SourceTracker determined 16S rRNA bacterial communites within freshwater samples, shared taxonomic similarities to that of wastewater at low levels (typically <3%). SourceTraker also predicted occasional fecal detection of deer and flying fox sources in the water samples. No significant differences in source contributions were observed within sequences from current and previously characterized fecal samples (P ≥ 0.107). However, significant differences were observed between previously characterized and newly characterized source communities (ANOSIM P ≤ 0.001), which shared <15% community composition. Results suggest temporal instability of fecal taxon libraries among tested sources and highlight continual evaluation of community-based MST using confirmatory qPCR analyses of marker genes.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofjournalWater Research
dc.relation.ispartofvolume165
dc.subject.fieldofresearchEnvironmental sciences
dc.subject.fieldofresearchcode41
dc.subject.keywordsScience & Technology
dc.subject.keywordsTechnology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsPhysical Sciences
dc.subject.keywordsEngineering, Environmental
dc.titleCompositional and temporal stability of fecal taxon libraries for use with SourceTracker in sub-tropical catchments
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationO'Dea, C; Zhang, Q; Staley, C; Masters, N; Kuballa, A; Fisher, P; Veal, C; Stratton, H; Sadowsky, MJ; Ahmed, W; Katouli, M, Compositional and temporal stability of fecal taxon libraries for use with SourceTracker in sub-tropical catchments, Water Research, 2019, 165
dcterms.dateAccepted2019-08-08
dc.date.updated2020-09-18T01:12:51Z
gro.hasfulltextNo Full Text
gro.griffith.authorStratton, Helen M.


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record