Virus-Derived Nanoparticles
Author(s)
Dashti, Noor H
Sainsbury, Frank
Griffith University Author(s)
Year published
2020
Metadata
Show full item recordAbstract
Capsid-based virus particles are widely engineered as viral nanoparticles and virus-like nanoparticles. The highly organized and uniform capsid structures make them ideal candidates for both in vitro and in vivo applications such as therapeutic delivery vehicles or enzymatic nanoreactors. Viruses have adapted to naturally infect a wide variety of organisms making their production achievable in various expression systems from bacterial to plants. Viral capsids can be modified externally and internally to suit the final application. The wide range of possible applications, ease of production in the system of choice, and ...
View more >Capsid-based virus particles are widely engineered as viral nanoparticles and virus-like nanoparticles. The highly organized and uniform capsid structures make them ideal candidates for both in vitro and in vivo applications such as therapeutic delivery vehicles or enzymatic nanoreactors. Viruses have adapted to naturally infect a wide variety of organisms making their production achievable in various expression systems from bacterial to plants. Viral capsids can be modified externally and internally to suit the final application. The wide range of possible applications, ease of production in the system of choice, and customizable modification of viral capsids makes them an attractive choice in the field of nanotechnology. In this chapter we aim to provide a generic protocol for the purification and characterization of virus-derived nanoparticles and methodology for chemically labelling them to monitor their uptake in mammalian cells.
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View more >Capsid-based virus particles are widely engineered as viral nanoparticles and virus-like nanoparticles. The highly organized and uniform capsid structures make them ideal candidates for both in vitro and in vivo applications such as therapeutic delivery vehicles or enzymatic nanoreactors. Viruses have adapted to naturally infect a wide variety of organisms making their production achievable in various expression systems from bacterial to plants. Viral capsids can be modified externally and internally to suit the final application. The wide range of possible applications, ease of production in the system of choice, and customizable modification of viral capsids makes them an attractive choice in the field of nanotechnology. In this chapter we aim to provide a generic protocol for the purification and characterization of virus-derived nanoparticles and methodology for chemically labelling them to monitor their uptake in mammalian cells.
View less >
Journal Title
Protein Nanotechnology: Protocols, Instrumentation, and Applications
Volume
2073
Subject
Other chemical sciences
Biochemistry and cell biology
Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
Nanoscience & Nanotechnology