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  • Virus-Derived Nanoparticles

    Author(s)
    Dashti, Noor H
    Sainsbury, Frank
    Griffith University Author(s)
    Sainsbury, Frank
    Year published
    2020
    Metadata
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    Abstract
    Capsid-based virus particles are widely engineered as viral nanoparticles and virus-like nanoparticles. The highly organized and uniform capsid structures make them ideal candidates for both in vitro and in vivo applications such as therapeutic delivery vehicles or enzymatic nanoreactors. Viruses have adapted to naturally infect a wide variety of organisms making their production achievable in various expression systems from bacterial to plants. Viral capsids can be modified externally and internally to suit the final application. The wide range of possible applications, ease of production in the system of choice, and ...
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    Capsid-based virus particles are widely engineered as viral nanoparticles and virus-like nanoparticles. The highly organized and uniform capsid structures make them ideal candidates for both in vitro and in vivo applications such as therapeutic delivery vehicles or enzymatic nanoreactors. Viruses have adapted to naturally infect a wide variety of organisms making their production achievable in various expression systems from bacterial to plants. Viral capsids can be modified externally and internally to suit the final application. The wide range of possible applications, ease of production in the system of choice, and customizable modification of viral capsids makes them an attractive choice in the field of nanotechnology. In this chapter we aim to provide a generic protocol for the purification and characterization of virus-derived nanoparticles and methodology for chemically labelling them to monitor their uptake in mammalian cells.
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    Journal Title
    Protein Nanotechnology: Protocols, Instrumentation, and Applications
    Volume
    2073
    DOI
    https://doi.org/10.1007/978-1-4939-9869-2_9
    Subject
    Other chemical sciences
    Biochemistry and cell biology
    Science & Technology
    Life Sciences & Biomedicine
    Biochemical Research Methods
    Biochemistry & Molecular Biology
    Nanoscience & Nanotechnology
    Publication URI
    http://hdl.handle.net/10072/397664
    Collection
    • Journal articles

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