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dc.contributor.authorArababadi, Mohammad K
dc.contributor.authorPourfathollah, Ali A
dc.contributor.authorJafarzadeh, Abdollah
dc.contributor.authorHassanshahi, Gholamhossein
dc.contributor.authorDaneshmandi, Saeed
dc.contributor.authorShamsizadeh, Ali
dc.contributor.authorKennedy, Derek
dc.date.accessioned2017-05-03T11:12:28Z
dc.date.available2017-05-03T11:12:28Z
dc.date.issued2011
dc.date.modified2011-08-09T06:27:26Z
dc.identifier.issn1319-3767
dc.identifier.doi10.4103/1319-3767.74461
dc.identifier.urihttp://hdl.handle.net/10072/39784
dc.description.abstractOccult hepatitis B infection (OBI) is identified as a form of hepatitis in which despite the absence of detectable HBsAg, HBV-DNA is observed in peripheral blood of patients. The main aim of this study has been to investigate the association between polymorphisms in +874 of IFN-? and +1188 of IL-12 with their serum level in patients suffering from OBI. Materials and Methods: In this experimental study, plasma samples of 3700 blood donors were tested for the presence of hepatitis B surface antigen (HBsAg) and anti-HBc by ELISA. The HBsAg-/anti-HBc+ samples were selected and screened for HBVDNA by PCR. HBV-DNA positive samples were assigned as OBI cases and ARMS-PCR techniques were performed to examine the two known polymorphisms within IL-12 and IFN-?. In addition, the serum levels of IL-12 and IFN-? were also determined by ELISA. Results: Results of this study demonstrated that, 352 (9.5%) out of 3700 blood samples were HBsAg-/anti-HBc+ and HBV-DNA was detected in 57/352 (16.1%) of HBsAg-/anti-HBc+ samples. Our results showed that groups showed signifi cant difference in CC allele of +1188 region of IL-12 and no difference was observed in the other evaluated genes. Our results also showed that the alleles of +1188 region of IL-12 and alleles of +874 of IFN-? were also not associated with serum level of cytokines. Conclusion: According to the results of this study, it may be concluded that the polymorphisms in +1188 region of IL-12 and +874 region of IFN-? would not affect the expression of both cytokines at serum level in OBI patients.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent679867 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoen_AU
dc.publisherMedknow Publications
dc.publisher.placeIndia
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom30
dc.relation.ispartofpageto35
dc.relation.ispartofissue1
dc.relation.ispartofjournalSaudi Journal of Gastroenterology
dc.relation.ispartofvolume17
dc.rights.retentionY
dc.subject.fieldofresearchMedical Genetics (excl. Cancer Genetics)
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchcode110311
dc.subject.fieldofresearchcode1103
dc.titleNon-association of IL-12 +1188 and IFN-γ +874 Polymorphisms with Cytokines Serum Level in Occult HBV Infected Patients
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2011 Saudi Gastroenterology Association (SGA). Published by Medknow. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.date.issued2011
gro.hasfulltextFull Text
gro.griffith.authorKennedy, Derek D.


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