Fluorescent Carbon Dots Functionalized with Self-Assembled Glycan Monolayers for Probing Interactions across the Glyco- Interactome
Author(s)
Cooper, Oren
Eftekhari, Ehsan
Carter, James
Mallard, Brody
Kaur, Jasreet
Kiefel, Milton J
Haselhorst, Thomas
Li, Qin
Tiralongo, Joe
Griffith University Author(s)
Year published
2020
Metadata
Show full item recordAbstract
Multivalent protein–glycan interactions are widespread in biology and are vital for initial recognition in cell–cell communication, host immune regulation, and viral and bacterial infection. Recently, fluorescent nanomaterials such as quantum dots (QDs) have been identified as a capable scaffold for multivalent carbohydrate immobilization. Carbon dots (CDs) are essentially heavy-metal-free QDs, offering a low-toxicity alternative for bio-imaging and biosensing applications. Herein, we report a simple and versatile route for linker functionalization of lactose with (3-glycidyloxypropyl)trimethoxysilane (GOPTS) for conjugation ...
View more >Multivalent protein–glycan interactions are widespread in biology and are vital for initial recognition in cell–cell communication, host immune regulation, and viral and bacterial infection. Recently, fluorescent nanomaterials such as quantum dots (QDs) have been identified as a capable scaffold for multivalent carbohydrate immobilization. Carbon dots (CDs) are essentially heavy-metal-free QDs, offering a low-toxicity alternative for bio-imaging and biosensing applications. Herein, we report a simple and versatile route for linker functionalization of lactose with (3-glycidyloxypropyl)trimethoxysilane (GOPTS) for conjugation to various CDs. CDs derived by thermal treatment of polyethylenimine (PEI) with citric acid and lactose, as the glycan, were employed in a number of detailed biological applications and evaluations. The self-assembled glycan monolayer (SAGM) method described here resulted in a high yield of lactose-conjugated CDs. Specific interactions of our lactose-coated CDs with cells, lectin microarrays, and as internalized bioimaging nanolights were demonstrated, with intracellular localization in a variety of cell lines observed. This present study offers a facile, one-pot, green, and low-cost synthesis of fluorescent multivalent nanoparticles that can be applied in the study of diverse interactions across the glyco-interactome.
View less >
View more >Multivalent protein–glycan interactions are widespread in biology and are vital for initial recognition in cell–cell communication, host immune regulation, and viral and bacterial infection. Recently, fluorescent nanomaterials such as quantum dots (QDs) have been identified as a capable scaffold for multivalent carbohydrate immobilization. Carbon dots (CDs) are essentially heavy-metal-free QDs, offering a low-toxicity alternative for bio-imaging and biosensing applications. Herein, we report a simple and versatile route for linker functionalization of lactose with (3-glycidyloxypropyl)trimethoxysilane (GOPTS) for conjugation to various CDs. CDs derived by thermal treatment of polyethylenimine (PEI) with citric acid and lactose, as the glycan, were employed in a number of detailed biological applications and evaluations. The self-assembled glycan monolayer (SAGM) method described here resulted in a high yield of lactose-conjugated CDs. Specific interactions of our lactose-coated CDs with cells, lectin microarrays, and as internalized bioimaging nanolights were demonstrated, with intracellular localization in a variety of cell lines observed. This present study offers a facile, one-pot, green, and low-cost synthesis of fluorescent multivalent nanoparticles that can be applied in the study of diverse interactions across the glyco-interactome.
View less >
Journal Title
ACS Applied Nano Materials
Volume
3
Issue
8
Subject
Biological sciences
Industrial biotechnology
Macromolecular and materials chemistry
Nanotechnology
Science & Technology
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Science & Technology - Other Topics