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dc.contributor.authorSaeed, Ayad
dc.contributor.authorUmer, Muhammad
dc.contributor.authorYamasaki, Naoki
dc.contributor.authorAzuma, Shinya
dc.contributor.authorUeda, Tadaharu
dc.contributor.authorShiddiky, Muhammad JA
dc.date.accessioned2020-09-30T01:27:54Z
dc.date.available2020-09-30T01:27:54Z
dc.date.issued2020
dc.identifier.issn2196-0216
dc.identifier.doi10.1002/celc.202000544
dc.identifier.urihttp://hdl.handle.net/10072/398004
dc.description.abstractThis article reports on the peroxidase-like catalytic activity of polyoxometalates (POMs) and their potential use as natural peroxidases for developing a simple and efficient colorimetric glucose sensor. Two Keggin-type vanadium-substituted tungstosulfates, [SVW11O40]3− (SVW11) and [SV2W10O40]4− (SV2W10), were tested for their potential as natural enzyme mimetics and exhibited strong peroxidase-like catalytic activity. The catalysis reaction was found to be in accordance with Michaelis-Menten and Lineweaver-Burk kinetics models. Michaelis-Menten constant (Km) and maximum velocity (Vmax) parameters were calculated to be 0.0759 mM and 0.329×10−8 Ms−1 for SVW11, and 0.0543 mM and 2.67×10−8 Ms−1 for SV2W10, respectively, indicating a high catalytic activity and a strong affinity of POMs towards 3,3,5,5-tetramethylbenzidine (TMB). In the case of H2O2, these values were found to be 57.1 mM and 0.325×10−8 mMs−1 for SVW11, and 47.7 mM and 2.72×10−8 mMs−1 for SV2W10. The peroxidase-like catalytic activity of these POMs was used to develop colorimetric glucose sensors as a proof-of-concept model for the POM-based naked-eye detection of biomolecules. The limit of detcetion (LOD) of glucose for SVW11 and SV2W10 was 1.14 μM and 1.24 μM, respectively. Our findings propose broad-ranging potential applications of these novel POMs in biosensing and bioanalytical chemistry.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofjournalChemElectroChem
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchPhysical chemistry
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode3406
dc.subject.fieldofresearchcode3499
dc.subject.keywordsScience & Technology
dc.subject.keywordsPhysical Sciences
dc.subject.keywordsElectrochemistry
dc.subject.keywordspolyoxometalates
dc.subject.keywordsperoxidase-like activity
dc.titleVanadium-Substituted Tungstosulfate Polyoxometalates as Peroxidase Mimetics and Their Potential Application in Biosensing
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationSaeed, A; Umer, M; Yamasaki, N; Azuma, S; Ueda, T; Shiddiky, MJA, Vanadium-Substituted Tungstosulfate Polyoxometalates as Peroxidase Mimetics and Their Potential Application in Biosensing, ChemElectroChem, 2020
dc.date.updated2020-09-29T01:43:04Z
dc.description.versionAccepted Manuscript (AM)
gro.description.notepublicThis publication has been entered in Griffith Research Online as an advanced online version.
gro.rights.copyright© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Vanadium‐Substituted Tungstosulfate Polyoxometalates as Peroxidase Mimetics and Their Potential Application in Biosensing, ChemElectroChem, 2020, which has been published in final form at https://doi.org/10.1002/celc.202000544. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)
gro.hasfulltextFull Text
gro.griffith.authorShiddiky, Muhammad J.
gro.griffith.authorUmer, Muhammad
gro.griffith.authorSaeed, Ayad A.


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