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dc.contributor.authorKasetsirikul, Surasak
dc.contributor.authorUmer, Muhammad
dc.contributor.authorSoda, Narshone
dc.contributor.authorRajan, Sreejith Kamalalayam
dc.contributor.authorShiddiky, Muhammad JA
dc.contributor.authorNguyen, Nam-Trung
dc.date.accessioned2020-09-29T02:03:32Z
dc.date.available2020-09-29T02:03:32Z
dc.date.issued2020
dc.identifier.issn0003-2654
dc.identifier.doi10.1039/d0an01609h
dc.identifier.urihttp://hdl.handle.net/10072/398008
dc.description.abstractThis work reports the development of a rapid, simple and inexpensive colorimetric paper-based assay for the detection of the severe acute respiratory symptom coronavirus 2 (SARS-CoV-2) humanized antibody. The paper device was prepared with lamination for easy sample handling and coated with the recombinant SARS-CoV-2 nucleocapsid antigen. This assay employed a colorimetric reaction, which is followed by horseradish peroxidase (HRP) conjugated detecting antibody in the presence of the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate. The colorimetric readout was evaluated and quantified for specificity and sensitivity. The characterization of this assay includes determining the linear regression curve, the limit of detection (LOD), the repeatability, and testing complex biological samples. We found that the LOD of the assay was 9.00 ng μL−1 (0.112 IU mL−1). The relative standard deviation was approximately 10% for a sample number of n = 3. We believe that our proof-of-concept assay has the potential to be developed for clinical screening of the SARS-CoV-2 humanized antibody as a tool to confirm infected active cases or to confirm SARS-CoV-2 immune cases during the process of vaccine development.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherRoyal Society of Chemistry (RSC)
dc.relation.ispartofjournalAnalyst
dc.subject.fieldofresearchAnalytical Chemistry
dc.subject.fieldofresearchOther Chemical Sciences
dc.subject.fieldofresearchcode0301
dc.subject.fieldofresearchcode0399
dc.titleDetection of SARS-CoV-2 humanized antibody with paper-based ELISA
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationKasetsirikul, S; Umer, M; Soda, N; Rajan, SK; Shiddiky, MJA; Nguyen, N-T, Detection of SARS-CoV-2 humanized antibody with paper-based ELISA, Analyst
dc.date.updated2020-09-29T01:59:26Z
dc.description.versionAccepted Manuscript (AM)
gro.description.notepublicThis publication has been entered in Griffith Research Online as an advanced online version.
gro.rights.copyright© 2020 Royal Society of Chemistry. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
gro.hasfulltextFull Text
gro.griffith.authorUmer, Muhammad
gro.griffith.authorKasetsirikul, Surasak
gro.griffith.authorSoda, Narshone
gro.griffith.authorKamalalayam Rajan, Sreejith
gro.griffith.authorShiddiky, Muhammad J.
gro.griffith.authorNguyen, Nam-Trung


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