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dc.contributor.authorGrutter, Alexandra S
dc.contributor.authorFeeney, William E
dc.contributor.authorHutson, Kate S
dc.contributor.authorMcClure, Eva C
dc.contributor.authorNarvaez, Pauline
dc.contributor.authorSmit, Nico J
dc.contributor.authorSun, Derek
dc.contributor.authorSikkel, Paul C
dc.date.accessioned2020-10-06T06:20:26Z
dc.date.available2020-10-06T06:20:26Z
dc.date.issued2020
dc.identifier.issn0020-7519
dc.identifier.doi10.1016/j.ijpara.2020.03.014
dc.identifier.urihttp://hdl.handle.net/10072/398129
dc.description.abstractThe reliance of parasites on their hosts makes host-parasite interactions ideal models for exploring ecological and evolutionary processes. By providing a consistent supply of parasites, in vivo monocultures offer the opportunity to conduct experiments on a scale that is generally not otherwise possible. Gnathiid isopods are common ectoparasites of marine fishes, and are becoming an increasing focus of research attention due to their experimental amenability and ecological importance as ubiquitous, harmful, blood-feeding "mosquito-like" organisms. They feed on hosts once during each of their three juvenile stages, and after each feeding event they return to the benthos to digest and moult to the next stage. Adults do not feed and remain in the benthos, where they reproduce and give birth. Here, we provide methods of culturing gnathiids, and highlight ways in which gnathiids can be used to examine parasite-host-environment interactions. Captive-raised gnathiid juveniles are increasingly being used in parasitological research; however, the methodology for establishing gnathiid monocultures is still not widely known. Information to obtain in vivo monocultures on teleost fish is detailed for a Great Barrier Reef (Australia) and a Caribbean Sea (US Virgin Islands) gnathiid species, and gnathiid information gained over two decades of successfully maintaining continuous cultures is summarised. Providing a suitable benthic habitat for the predominantly benthic free-living stage of this parasite is paramount. Maintenance comprises provision of adequate benthic shelter, managing parasite populations, and sustaining host health. For the first time, we also measured gnathiids' apparent attack speed (maximum 24.5 cm sec-1; 6.9, 4.9/17.0, median, 25th/75th quantiles) and illustrate how to collect such fast moving ectoparasites in captivity for experiments. In addition to providing details pertaining to culture maintenance, we review research using gnathiid cultures that have enabled detailed scientific understanding of host and parasite biology, behaviour and ecology on coral reefs.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom825
dc.relation.ispartofpageto837
dc.relation.ispartofissue10-11
dc.relation.ispartofjournalInternational Journal for Parasitology
dc.relation.ispartofvolume50
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchZoology
dc.subject.fieldofresearchVeterinary Sciences
dc.subject.fieldofresearchcode0605
dc.subject.fieldofresearchcode0608
dc.subject.fieldofresearchcode0707
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsParasitology
dc.subject.keywordsGnathiidae
dc.subject.keywordsMonoculture
dc.titlePractical methods for culturing parasitic gnathiid isopods
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationGrutter, AS; Feeney, WE; Hutson, KS; McClure, EC; Narvaez, P; Smit, NJ; Sun, D; Sikkel, PC, Practical methods for culturing parasitic gnathiid isopods, Practical methods for culturing parasitic gnathiid isopods, 2020, 50 (10-11), pp. 825-837
dcterms.dateAccepted2020-03-17
dc.date.updated2020-10-06T01:59:50Z
gro.hasfulltextNo Full Text
gro.griffith.authorMcClure, Eva
gro.griffith.authorFeeney, William


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