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dc.contributor.authorAbbasi, Naghmeh
dc.contributor.authorLee, Ryan SB
dc.contributor.authorIvanovski, Saso
dc.contributor.authorLove, Robert M
dc.contributor.authorHamlet, Stephen
dc.date.accessioned2020-10-08T02:40:14Z
dc.date.available2020-10-08T02:40:14Z
dc.date.issued2020
dc.identifier.issn1226-4601
dc.identifier.doi10.1186/s40824-020-00196-1
dc.identifier.urihttp://hdl.handle.net/10072/398208
dc.description.abstractBackground: Biomaterial-based bone tissue engineering represents a promising solution to overcome reduced residual bone volume. It has been previously demonstrated that gradient and offset architectures of three-dimensional melt electrowritten poly-caprolactone (PCL) scaffolds could successfully direct osteoblast cells differentiation toward an osteogenic lineage, resulting in mineralization. The aim of this study was therefore to evaluate the in vivo osteoconductive capacity of PCL scaffolds with these different architectures. Methods: Five different calcium phosphate (CaP) coated melt electrowritten PCL pore sized scaffolds: 250 μm and 500 μm, 500 μm with 50% fibre offset (offset.50.50), tri layer gradient 250-500-750 μm (grad.250top) and 750-500-250 μm (grad.750top) were implanted into rodent critical-sized calvarial defects. Empty defects were used as a control. After 4 and 8 weeks of healing, the new bone was assessed by micro-computed tomography and immunohistochemistry. Results: Significantly more newly formed bone was shown in the grad.250top scaffold 8 weeks post-implantation. Histological investigation also showed that soft tissue was replaced with newly formed bone and fully covered the grad.250top scaffold. While, the bone healing did not happen completely in the 250 μm, offset.50.50 scaffolds and blank calvaria defects following 8 weeks of implantation. Immunohistochemical analysis showed the expression of osteogenic markers was present in all scaffold groups at both time points. The mineralization marker Osteocalcin was detected with the highest intensity in the grad.250top and 500 μm scaffolds. Moreover, the expression of the endothelial markers showed that robust angiogenesis was involved in the repair process. Conclusions: These results suggest that the gradient pore size structure provides superior conditions for bone regeneration.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer Science and Business Media LLC
dc.relation.ispartofpagefrom17
dc.relation.ispartofissue1
dc.relation.ispartofjournalBiomaterials Research
dc.relation.ispartofvolume24
dc.subject.fieldofresearchBiomedical engineering
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchMacromolecular and materials chemistry
dc.subject.fieldofresearchcode4003
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode3403
dc.subject.keywordsAngiogenesis
dc.subject.keywordsBone tissue engineering
dc.subject.keywordsMelt electrowriting
dc.subject.keywordsPoly (ε-caprolactone)
dc.subject.keywordsPore size
dc.titleIn vivo bone regeneration assessment of offset and gradient melt electrowritten (MEW) PCL scaffolds
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationAbbasi, N; Lee, RSB; Ivanovski, S; Love, RM; Hamlet, S, In vivo bone regeneration assessment of offset and gradient melt electrowritten (MEW) PCL scaffolds, Biomaterials Research, 2020, 24 (1), pp. 17
dcterms.dateAccepted2020-09-21
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2020-10-08T00:34:44Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s). 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data
gro.hasfulltextFull Text
gro.griffith.authorHamlet, Stephen


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