dc.contributor.author | Bouquet, M | |
dc.contributor.author | Passmore, MR | |
dc.contributor.author | See Hoe, LE | |
dc.contributor.author | Tung, JP | |
dc.contributor.author | Simonova, G | |
dc.contributor.author | Boon, AC | |
dc.contributor.author | Fraser, JF | |
dc.date.accessioned | 2020-11-04T04:34:41Z | |
dc.date.available | 2020-11-04T04:34:41Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 0022-1759 | |
dc.identifier.doi | 10.1016/j.jim.2020.112835 | |
dc.identifier.uri | http://hdl.handle.net/10072/398965 | |
dc.description.abstract | There is growing evidence that inflammation underpins many common diseases. Inflammatory/immunomodulatory/immune mediators, such as cytokines, are key modulators of inflammation and mediate both immune cell recruitment and complex intracellular signalling pathways. Ovine models of disease are increasingly utilized in pre-clinical research, however existing methods for measuring cytokine levels are limited. We established and validated enzyme-linked immunosorbent assays (ELISAs) targeting interleukin (IL)-1β, IL-6, IL-8 and IL-10 in sheep plasma. These ELISAs showed high sensitivity and specificity with intra- and inter-assay CV's below 10%, and recovery rates between 82 and 123%. Sensitivity for IL-1β, IL-6, IL-8 and IL-10 were 117.6 pg/mL, 443.1 pg/mL, 30.9 pg/mL, and 64.3 pg/mL, respectively. ELISA test result reproducibility decreased significantly after 12 weeks of plasma storage at −80 °C. Therefore, for accurate cytokine measurements, plasma samples need to be tested within three months of sample collection to account for cytokine protein degradation. These ELISAs offer a reliable and convenient method to identify inflammatory cytokine changes in sheep, allowing key insights into the disease pathogenesis of these ruminants. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Elsevier | |
dc.relation.ispartofpagefrom | 112835 | |
dc.relation.ispartofjournal | Journal of Immunological Methods | |
dc.relation.ispartofvolume | 486 | |
dc.subject.fieldofresearch | Immunology | |
dc.subject.fieldofresearch | Medical microbiology | |
dc.subject.fieldofresearch | Ophthalmology and optometry | |
dc.subject.fieldofresearchcode | 3204 | |
dc.subject.fieldofresearchcode | 3207 | |
dc.subject.fieldofresearchcode | 3212 | |
dc.subject.keywords | Cytokines | |
dc.subject.keywords | ELISA | |
dc.subject.keywords | Inflammation | |
dc.subject.keywords | Sheep | |
dc.title | Development and validation of ELISAs for the quantitation of interleukin (IL)-1β, IL-6, IL-8 and IL-10 in ovine plasma | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dcterms.bibliographicCitation | Bouquet, M; Passmore, MR; See Hoe, LE; Tung, JP; Simonova, G; Boon, AC; Fraser, JF, Development and validation of ELISAs for the quantitation of interleukin (IL)-1β, IL-6, IL-8 and IL-10 in ovine plasma, Journal of Immunological Methods, 2020, 486, pp. 112835- | |
dcterms.dateAccepted | 2020-08-12 | |
dc.date.updated | 2020-11-04T04:30:31Z | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Fraser, John F. | |
gro.griffith.author | See Hoe, Louise | |