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dc.contributor.authorBouquet, M
dc.contributor.authorPassmore, MR
dc.contributor.authorSee Hoe, LE
dc.contributor.authorTung, JP
dc.contributor.authorSimonova, G
dc.contributor.authorBoon, AC
dc.contributor.authorFraser, JF
dc.date.accessioned2020-11-04T04:34:41Z
dc.date.available2020-11-04T04:34:41Z
dc.date.issued2020
dc.identifier.issn0022-1759
dc.identifier.doi10.1016/j.jim.2020.112835
dc.identifier.urihttp://hdl.handle.net/10072/398965
dc.description.abstractThere is growing evidence that inflammation underpins many common diseases. Inflammatory/immunomodulatory/immune mediators, such as cytokines, are key modulators of inflammation and mediate both immune cell recruitment and complex intracellular signalling pathways. Ovine models of disease are increasingly utilized in pre-clinical research, however existing methods for measuring cytokine levels are limited. We established and validated enzyme-linked immunosorbent assays (ELISAs) targeting interleukin (IL)-1β, IL-6, IL-8 and IL-10 in sheep plasma. These ELISAs showed high sensitivity and specificity with intra- and inter-assay CV's below 10%, and recovery rates between 82 and 123%. Sensitivity for IL-1β, IL-6, IL-8 and IL-10 were 117.6 pg/mL, 443.1 pg/mL, 30.9 pg/mL, and 64.3 pg/mL, respectively. ELISA test result reproducibility decreased significantly after 12 weeks of plasma storage at −80 °C. Therefore, for accurate cytokine measurements, plasma samples need to be tested within three months of sample collection to account for cytokine protein degradation. These ELISAs offer a reliable and convenient method to identify inflammatory cytokine changes in sheep, allowing key insights into the disease pathogenesis of these ruminants.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom112835
dc.relation.ispartofjournalJournal of Immunological Methods
dc.relation.ispartofvolume486
dc.subject.fieldofresearchImmunology
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchOphthalmology and optometry
dc.subject.fieldofresearchcode3204
dc.subject.fieldofresearchcode3207
dc.subject.fieldofresearchcode3212
dc.subject.keywordsCytokines
dc.subject.keywordsELISA
dc.subject.keywordsInflammation
dc.subject.keywordsSheep
dc.titleDevelopment and validation of ELISAs for the quantitation of interleukin (IL)-1β, IL-6, IL-8 and IL-10 in ovine plasma
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationBouquet, M; Passmore, MR; See Hoe, LE; Tung, JP; Simonova, G; Boon, AC; Fraser, JF, Development and validation of ELISAs for the quantitation of interleukin (IL)-1β, IL-6, IL-8 and IL-10 in ovine plasma, Journal of Immunological Methods, 2020, 486, pp. 112835-
dcterms.dateAccepted2020-08-12
dc.date.updated2020-11-04T04:30:31Z
gro.hasfulltextNo Full Text
gro.griffith.authorFraser, John F.
gro.griffith.authorSee Hoe, Louise


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