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dc.contributor.authorNguyen, Nhat-Khuong
dc.contributor.authorSingha, Pradip
dc.contributor.authorZhang, Jun
dc.contributor.authorPhan, Hoang-Phuong
dc.contributor.authorNguyen, Nam-Trung
dc.contributor.authorOoi, Chin Hong
dc.date.accessioned2020-11-11T03:25:57Z
dc.date.available2020-11-11T03:25:57Z
dc.date.issued2020
dc.identifier.issn1439-4235
dc.identifier.doi10.1002/cphc.202000760
dc.identifier.urihttp://hdl.handle.net/10072/399207
dc.description.abstractLiquid marble is a promising microreactor platform that recently attracts significant research interest owing to their ability to accommodate a wide range of micro reactions. However, the use of destructive and ex-situ methods to monitor reactions impairs the potential of liquid-marble-based microreactors. This paper proposes a non-destructive, in situ, and cost-effective digital-imaging-based colourimetric monitoring method for transparent liquid marbles, with the example of the enzymatic hydrolysis of starch. The colourimetric reaction between starch and iodine produces a complex that exhibits a dark blue colour. We found that the absorbance of red channel of digital images showed a linear relationship with starch concentration with high sensitivity and repeatability. This digital-imaging based-colourimetric method was used to study the hydrolysis of starch by α-amylase. The results showed high accuracy and applicability of first-order kinetic for this reaction. The demonstration of digital-imaging-based colourimetry indicates the potential of liquid marble-based microreactors.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofjournalChemPhysChem
dc.subject.fieldofresearchAtomic, Molecular, Nuclear, Particle and Plasma Physics
dc.subject.fieldofresearchPhysical Chemistry (incl. Structural)
dc.subject.fieldofresearchTheoretical and Computational Chemistry
dc.subject.fieldofresearchcode0202
dc.subject.fieldofresearchcode0306
dc.subject.fieldofresearchcode0307
dc.subject.keywordscolourimetry
dc.subject.keywordsdigital imaging
dc.subject.keywordsliquid marble
dc.subject.keywordsmicroreactor
dc.subject.keywordsstarch hydrolysis
dc.titleDigital imaging-based colourimetry for enzymatic processes in transparent liquid marbles.
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationNguyen, N-K; Singha, P; Zhang, J; Phan, H-P; Nguyen, N-T; Ooi, CH, Digital imaging-based colourimetry for enzymatic processes in transparent liquid marbles., ChemPhysChem, 2020
dcterms.dateAccepted2020-11-06
dc.date.updated2020-11-11T03:10:06Z
dc.description.versionAccepted Manuscript (AM)
gro.rights.copyright© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Digital imaging‐based colourimetry for enzymatic processes in transparent liquid marbles, ChemPhysChem, 2020, which has been published in final form at https://doi.org/10.1002/cphc.202000760. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)
gro.hasfulltextFull Text
gro.griffith.authorSingha, Pradip
gro.griffith.authorNguyen, Nhat Khuong
gro.griffith.authorPhan, Hoang Phuong
gro.griffith.authorNguyen, Nam-Trung
gro.griffith.authorZhang, Jun
gro.griffith.authorOoi, Chin Hong


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