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dc.contributor.authorWoodberry, Tonia
dc.contributor.authorPinzon-Charry, Alberto
dc.contributor.authorPiera, Kim A
dc.contributor.authorPanpisutchai, Yawalak
dc.contributor.authorEngwerda, Christian R
dc.contributor.authorDoolan, Denise L
dc.contributor.authorSalwati, Ervi
dc.contributor.authorKenangalem, Enny
dc.contributor.authorTjitra, Emiliana
dc.contributor.authorPrice, Ric N
dc.contributor.authorGood, Michael F
dc.contributor.authorAnstey, Nicholas M
dc.date.accessioned2018-01-11T03:51:12Z
dc.date.available2018-01-11T03:51:12Z
dc.date.issued2009
dc.date.modified2011-08-12T06:21:22Z
dc.identifier.issn1475-2875
dc.identifier.doi10.1186/1475-2875-8-122
dc.identifier.urihttp://hdl.handle.net/10072/39959
dc.description.abstractBackground The Plasmodium purine salvage enzyme, hypoxanthine guanine xanthine phosphoribosyl transferase (HGXPRT) can protect mice against Plasmodium yoelii pRBC challenge in a T cell-dependent manner and has, therefore, been proposed as a novel vaccine candidate. It is not known whether natural exposure to Plasmodium falciparum stimulates HGXPRT T cell reactivity in humans. Methods PBMC and plasma collected from malaria-exposed Indonesians during infection and 7-28 days after anti-malarial therapy, were assessed for HGXPRT recognition using CFSE proliferation, IFN? ELISPOT assay and ELISA. Results HGXPRT-specific T cell proliferation was found in 44% of patients during acute infection; in 80% of responders both CD4+ and CD8+ T cell subsets proliferated. Antigen-specific T cell proliferation was largely lost within 28 days of parasite clearance. HGXPRT-specific IFN-? production was more frequent 28 days after treatment than during acute infection. HGXPRT-specific plasma IgG was undetectable even in individuals exposed to malaria for at least two years. Conclusion The prevalence of acute proliferative and convalescent IFN? responses to HGXPRT demonstrates cellular immunogenicity in humans. Further studies to determine minimal HGXPRT epitopes, the specificity of responses for Plasmodia and associations with protection are required. Frequent and robust T cell proliferation, high sequence conservation among Plasmodium species and absent IgG responses distinguish HGXPRT from other malaria antigens.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoen_AU
dc.publisherBioMed Central Ltd.
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto10
dc.relation.ispartofjournalMalaria Journal
dc.relation.ispartofvolume8
dc.rights.retentionY
dc.subject.fieldofresearchMedical Microbiology not elsewhere classified
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchMedical Microbiology
dc.subject.fieldofresearchPublic Health and Health Services
dc.subject.fieldofresearchcode110899
dc.subject.fieldofresearchcode0605
dc.subject.fieldofresearchcode1108
dc.subject.fieldofresearchcode1117
dc.titleHuman T cell recognition of the blood stage antigen Plasmodium hypoxanthine guanine xanthine phosphoribosyl transferase (HGXPRT) in acute malaria
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/2.0
dc.description.versionPublished
gro.rights.copyright© 2009 Woodberry et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.date.issued2009
gro.hasfulltextFull Text
gro.griffith.authorGood, Michael F.
gro.griffith.authorPinzon-Charry, Alberto
gro.griffith.authorEngwerda, Christian R.


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