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dc.contributor.authorWoodberry, Toniaen_US
dc.contributor.authorPinzon-Charry, Albertoen_US
dc.contributor.authorPiera, Kimen_US
dc.contributor.authorPanpisutchai, Yawalaken_US
dc.contributor.authorEngwerda, Christianen_US
dc.contributor.authorDoolan, Deniseen_US
dc.contributor.authorSalwati, Ervien_US
dc.contributor.authorKenangalem, Ennyen_US
dc.contributor.authorTjitra, Emilianaen_US
dc.contributor.authorPrice, Ricen_US
dc.contributor.authorGood, Michaelen_US
dc.contributor.authorAnstey, Nicholasen_US
dc.description.abstractBackground The Plasmodium purine salvage enzyme, hypoxanthine guanine xanthine phosphoribosyl transferase (HGXPRT) can protect mice against Plasmodium yoelii pRBC challenge in a T cell-dependent manner and has, therefore, been proposed as a novel vaccine candidate. It is not known whether natural exposure to Plasmodium falciparum stimulates HGXPRT T cell reactivity in humans. Methods PBMC and plasma collected from malaria-exposed Indonesians during infection and 7-28 days after anti-malarial therapy, were assessed for HGXPRT recognition using CFSE proliferation, IFN? ELISPOT assay and ELISA. Results HGXPRT-specific T cell proliferation was found in 44% of patients during acute infection; in 80% of responders both CD4+ and CD8+ T cell subsets proliferated. Antigen-specific T cell proliferation was largely lost within 28 days of parasite clearance. HGXPRT-specific IFN-? production was more frequent 28 days after treatment than during acute infection. HGXPRT-specific plasma IgG was undetectable even in individuals exposed to malaria for at least two years. Conclusion The prevalence of acute proliferative and convalescent IFN? responses to HGXPRT demonstrates cellular immunogenicity in humans. Further studies to determine minimal HGXPRT epitopes, the specificity of responses for Plasmodia and associations with protection are required. Frequent and robust T cell proliferation, high sequence conservation among Plasmodium species and absent IgG responses distinguish HGXPRT from other malaria antigens.en_US
dc.publisherBioMed Central Ltd.en_US
dc.publisher.placeUnited Kingdomen_US
dc.relation.ispartofjournalMalaria Journalen_US
dc.subject.fieldofresearchMedical Microbiology not elsewhere classifieden_US
dc.titleHuman T cell recognition of the blood stage antigen Plasmodium hypoxanthine guanine xanthine phosphoribosyl transferase (HGXPRT) in acute malariaen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.hasfulltextNo Full Text

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