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dc.contributor.authorMills, Jamie-Lee S
dc.contributor.authorJayashi, Cesar M Flores
dc.contributor.authorReynolds, Simone
dc.contributor.authorWun, Christine
dc.contributor.authorCalcutt, Ainslie
dc.contributor.authorBen Baker, S
dc.contributor.authorMurugappan, Senthil
dc.contributor.authorDepelsenaire, Alexandra C
dc.contributor.authorDooley, Jessica
dc.contributor.authorFahey, Paul
dc.contributor.authorForster, Angus H
dc.contributor.authorPandey, Manisha
dc.contributor.authorGood, Michael F
dc.date.accessioned2020-11-27T01:32:42Z
dc.date.available2020-11-27T01:32:42Z
dc.date.issued2020
dc.identifier.issn2059-0105
dc.identifier.doi10.1038/s41541-020-00222-2
dc.identifier.urihttp://hdl.handle.net/10072/399718
dc.description.abstractWe evaluated vaccination against Streptococcus pyogenes with the candidate vaccine, J8-DT, delivered by a high-density microarray patch (HD-MAP). We showed that vaccination with J8-DT eluted from a coated HD-MAP (J8-DT/HD-MAP), induced similar total IgG responses to that generated by vaccination with J8-DT adjuvanted with Alum (J8-DT/Alum). We evaluated the effect of dose reduction and the number of vaccinations on the antibody response profile of vaccinated mice. A reduction in the number of vaccinations (from three to two) with J8-DT/HD-MAP induced comparable antibody responses to three vaccinations with intramuscular J8-DT/Alum. Vaccine-induced protection against an S. pyogenes skin challenge was assessed. J8-DT/HD-MAP vaccination led to a significant reduction in the number of S. pyogenes colony forming units in skin (92.9%) and blood (100%) compared to intramuscular vaccination with unadjuvanted J8-DT. The protection profile was comparable to that of intramuscular J8-DT/Alum. J8-DT/HD-MAP induced a shift in the antibody isotype profile, with a bias towards Th1-related isotypes, compared to J8-DT/Alum (Th2 bias). Based on the results of this study, the use of J8-DT/HD-MAP should be considered in future clinical development and control programs against S. pyogenes. Furthermore, the innate characteristics of the technology, such as vaccine stability and increased coverage, ease of use, reduction of sharp waste and the potential reduction of dose may be advantageous compared to current vaccination methods.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofpagefrom74
dc.relation.ispartofissue1
dc.relation.ispartofjournalnpj Vaccines
dc.relation.ispartofvolume5
dc.subject.fieldofresearchImmunology
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchcode3204
dc.subject.fieldofresearchcode3207
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsImmunology
dc.subject.keywordsMedicine, Research & Experimental
dc.subject.keywordsResearch & Experimental Medicine
dc.titleM-protein based vaccine induces immunogenicity and protection fromStreptococcus pyogeneswhen delivered on a high-density microarray patch (HD-MAP)
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationMills, J-LS; Jayashi, CMF; Reynolds, S; Wun, C; Calcutt, A; Ben Baker, S; Murugappan, S; Depelsenaire, AC; Dooley, J; Fahey, P; Forster, AH; Pandey, M; Good, MF, M-protein based vaccine induces immunogenicity and protection fromStreptococcus pyogeneswhen delivered on a high-density microarray patch (HD-MAP), npj Vaccines, 2020, 5 (1), pp. 74
dcterms.dateAccepted2020-07-10
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2020-11-27T01:27:52Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
gro.hasfulltextFull Text
gro.griffith.authorPandey, Manisha


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