• myGriffith
    • Staff portal
    • Contact Us⌄
      • Future student enquiries 1800 677 728
      • Current student enquiries 1800 154 055
      • International enquiries +61 7 3735 6425
      • General enquiries 07 3735 7111
      • Online enquiries
      • Staff phonebook
    View Item 
    •   Home
    • Griffith Research Online
    • Journal articles
    • View Item
    • Home
    • Griffith Research Online
    • Journal articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

  • All of Griffith Research Online
    • Communities & Collections
    • Authors
    • By Issue Date
    • Titles
  • This Collection
    • Authors
    • By Issue Date
    • Titles
  • Statistics

  • Most Popular Items
  • Statistics by Country
  • Most Popular Authors
  • Support

  • Contact us
  • FAQs
  • Admin login

  • Login
  • Zinc(II) complexes of indole thiosemicarbazones: DNA/protein binding, molecular docking and in vitro cytotoxicity studies

    Author(s)
    Balakrishnan, N
    Haribabu, J
    Anantha Krishnan, D
    Swaminathan, S
    Mahendiran, D
    Bhuvanesh, NSP
    Karvembu, R
    Griffith University Author(s)
    Dharmasivam, Mahendiran
    Year published
    2019
    Metadata
    Show full item record
    Abstract
    Four Zn(II) complexes (1–4) featuring indole thiosemicarbazones were synthesized and well-characterized by elemental analyses and various spectroscopic (UV–Vis, FT-IR, 1H NMR, 13C NMR and mass) techniques. The single crystal X-ray crystallographic study revealed a tetrahedral geometry for complexes 1 and 3. The interactions of the Zn(II) complexes 1–4 with calf thymus DNA (CT DNA) were examined by UV–Vis spectroscopy and viscosity measurements, which implied that the complexes bound to CT DNA via intercalation, and complex 4 showed a higher binding affinity than the other complexes. The protein binding interaction of the ...
    View more >
    Four Zn(II) complexes (1–4) featuring indole thiosemicarbazones were synthesized and well-characterized by elemental analyses and various spectroscopic (UV–Vis, FT-IR, 1H NMR, 13C NMR and mass) techniques. The single crystal X-ray crystallographic study revealed a tetrahedral geometry for complexes 1 and 3. The interactions of the Zn(II) complexes 1–4 with calf thymus DNA (CT DNA) were examined by UV–Vis spectroscopy and viscosity measurements, which implied that the complexes bound to CT DNA via intercalation, and complex 4 showed a higher binding affinity than the other complexes. The protein binding interaction of the complexes was monitored by fluorescence and absorption techniques, which showed that the complexes could bind effectively with bovine serum albumin (BSA) and the type of quenching mechanism was found to be static. Synchronous fluorescence experiments showed the changes in the conformations of the protein micro regions. Molecular docking studies were performed in order to get a better picture of the binding of the complexes with the molecular targets DNA hexamer and BSA. The in vitro cytotoxicity of the complexes against two human cancer (A549 and MCF7) cell lines, two human non-tumorigenic (MCF-10A and HEK-293) cell lines and one non-cancerous mouse fibroblasts (L929) cell line was evaluated using an MTT assay. Complex 4, which has an N-terminal cyclohexyl group, showed moderate activity [IC50 = 37.9 (A549) and 60.3 μM (MCF7)] that was comparable with the familiar anticancer drug cisplatin. Also, fortunately, the activity of complex 4 was found to be specific to cancer cells. The apoptosis cell death mechanism for complex 4 was assessed by the Hoechst staining method.
    View less >
    Journal Title
    Polyhedron
    Volume
    170
    DOI
    https://doi.org/10.1016/j.poly.2019.05.039
    Subject
    Inorganic chemistry
    Physical chemistry
    Other chemical sciences
    Publication URI
    http://hdl.handle.net/10072/399953
    Collection
    • Journal articles

    Footer

    Disclaimer

    • Privacy policy
    • Copyright matters
    • CRICOS Provider - 00233E
    • TEQSA: PRV12076

    Tagline

    • Gold Coast
    • Logan
    • Brisbane - Queensland, Australia
    First Peoples of Australia
    • Aboriginal
    • Torres Strait Islander