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dc.contributor.authorIqbal, S
dc.contributor.authorParker, LM
dc.contributor.authorEverest-Dass, AV
dc.contributor.authorMoh, ESX
dc.contributor.authorSayyadi, N
dc.contributor.authorHutchinson, MR
dc.contributor.authorPacker, NH
dc.description.abstractPolysialic acid (polySia), a long homopolymer of 2,8-linked sialic acids, is abundant in the embryonic brain and is restricted largely in adult brain to regions that exhibit neurogenesis and structural plasticity. In the central nervous system (CNS), polySia is highly important for cell-cell interactions, differentiation, migration and cytokine responses, which are critical neuronal functions regulating intercellular interactions that underlie immune signalling in the CNS. In recent reports, a metabolite of morphine, morphine-3-glucuronide (M3G), has been shown to cause immune signalling in the CNS. In this study, we compared the effects of neurite growth factor (NGF), lipopolysaccharide (LPS) and M3G exposure on the expression of polySia in PC12 cells using immunocytochemistry and Western blot analysis. PolySia was also extracted from stimulated cell proteins by endo-neuraminidase digestion and quantitated using fluorescent labelling followed by HPLC analysis. PolySia expression was significantly increased following NGF, M3G or LPS stimulation when compared with unstimulated cells or cells exposed to the TLR4 antagonist LPS-RS. Additionally, we analyzed the effects of test agent exposure on cell migration and the oxidative stress response of these cells in the presence and absence of polySia expression on their cell surface. We observed an increase in oxidative stress in cells without polySia as well as following M3G or LPS stimulation. Our study provides evidence that polySia expression in neuronal-like PC12 cells is influenced by M3G and LPS exposure alike, suggestive of a role of TLR4 in triggering these events.
dc.publisherSpringer Science and Business Media LLC
dc.relation.ispartofjournalMolecular Neurobiology
dc.subject.fieldofresearchCognitive Sciences
dc.subject.keywordsCentral nervous system
dc.subject.keywordsLipopolysaccharide (LPS)
dc.subject.keywordsMorphine-3-glucuronide (M3G)
dc.titleLipopolysaccharide and Morphine-3-Glucuronide-Induced Immune Signalling Increases the Expression of Polysialic Acid in PC12 Cells
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationIqbal, S; Parker, LM; Everest-Dass, AV; Moh, ESX; Sayyadi, N; Hutchinson, MR; Packer, NH, Lipopolysaccharide and Morphine-3-Glucuronide-Induced Immune Signalling Increases the Expression of Polysialic Acid in PC12 Cells, Molecular Neurobiology, 2020, 57 (2), pp. 964-975
gro.description.notepublicThis publication has been entered as an advanced online version in Griffith Research Online.
gro.hasfulltextNo Full Text
gro.griffith.authorEverest-Dass, Arun
gro.griffith.authorPacker, Nicki

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