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dc.contributor.authorAktar, S
dc.contributor.authorDas, PK
dc.contributor.authorAsha, SY
dc.contributor.authorSiddika, MA
dc.contributor.authorIslam, F
dc.contributor.authorKhanam, JA
dc.contributor.authorRakib, MA
dc.date.accessioned2020-12-07T02:35:17Z
dc.date.available2020-12-07T02:35:17Z
dc.date.issued2019
dc.identifier.issn2616-4760
dc.identifier.doi10.5455/jabet.2019.d28
dc.identifier.urihttp://hdl.handle.net/10072/400006
dc.description.abstractAngiotensin Converting Enzyme (ACE) regulates blood pressure. ACE converts angiotensin I to angiotensin II which binds with its receptors and through a cascade of reactions constrict blood vesels, consequently results in increased blood pressure, called hypertension. Inhibition of ACE activity is considered as an useful therapeutic target that reduce hypertension. Moringa oleifera leaves have traditionally been used in Ayurvedic medicine for their antihypertensive activity and antihypertensive effect of Moringa leaves was reported on spontaneously hypertensive rats. So, we hypothesize that Moringa oleifera leaves methanolic extract (MOLME) might inhibit ACE activity. Inhibition of ACE activity by MOLME was estimated in vitro. In this study, inhibition of ACE by MOLME was evaluated by spectrophotometric method. MOLME inhibited ACE activity in the substrate hippuryl-L-histidyl-L-leucine (HHL) with an IC50 value of 226.37 μg/ml with a reference compound, captropril (CP), a potent ACE inhibitor with an IC50 value of 0.0289 μM. The mode of ACE inhibition in HHL with or without MOLME revealed that the Vmax (0.0857 and 0.0541 OD/30 min, respectively) was changed and the Km values were 4.671 and 4.41. The results indicate that MOLME acts as a non-competitive inhibitor for ACE. CP was found a competitive inhibitor of ACE. MOLME might be a potential natural inhibitor of ACE which reduces hypertension.
dc.description.peerreviewedYes
dc.publisherBangladesh Society for Microbiology, Immunology, and Advanced Biotechnology (BSMIAB)
dc.relation.ispartofpagefrom73
dc.relation.ispartofpageto77
dc.relation.ispartofissue2
dc.relation.ispartofjournalJournal of Advanced Biotechnology and Experimental Therapeutics
dc.relation.ispartofvolume2
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchcode3202
dc.titleMoringa oleifera leaves methanolic extract inhibits angiotensin converting enzyme activity in vitro which ameliorates hypertension
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationAktar, S; Das, PK; Asha, SY; Siddika, MA; Islam, F; Khanam, JA; Rakib, MA, Moringa oleifera leaves methanolic extract inhibits angiotensin converting enzyme activity in vitro which ameliorates hypertension, Journal of Advanced Biotechnology and Experimental Therapeutics, 2019, 2 (2), pp. 73-77
dcterms.licensehttp://creativecommons.org/licenses/by-nc/4.0/
dc.date.updated2020-12-07T02:30:46Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2019. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorIslam, Farhad


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