Internal validation of STRmix™ - A multi laboratory response to PCAST

Bright, Jo-Anne; Richards, Rebecca; Kruijver, Maarten; Kelly, Hannah; McGovern, Catherine; Magee, Alan; McWhorter, Andrew; Ciecko, Anne; Peck, Brian; Baumgartner, Chase; Buettner, Christina; McWilliams, Scott; McKenna, Claire; Oefelein, Rachel H; et al.

doi:10.1016/j.fsigen.2018.01.003

Author(s)

Bright, Jo-Anne

Richards, Rebecca

Kruijver, Maarten

Kelly, Hannah

McGovern, Catherine

Magee, Alan

McWhorter, Andrew

Ciecko, Anne

Peck, Brian

Baumgartner, Chase

Buettner, Christina

McWilliams, Scott

McKenna, Claire

Oefelein, Rachel H

et al.

Griffith University Author(s)

Oefelein, Rachel H.

Year published

2018

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Abstract

We report a large compilation of the internal validations of the probabilistic genotyping software STRmix™. Thirty one laboratories contributed data resulting in 2825 mixtures comprising three to six donors and a wide range of multiplex, equipment, mixture proportions and templates. Previously reported trends in the LR were confirmed including less discriminatory LRs occurring both for donors and non-donors at low template (for the donor in question) and at high contributor number. We were unable to isolate an effect of allelic sharing. Any apparent effect appears to be largely confounded with increased contributor number.We report a large compilation of the internal validations of the probabilistic genotyping software STRmix™. Thirty one laboratories contributed data resulting in 2825 mixtures comprising three to six donors and a wide range of multiplex, equipment, mixture proportions and templates. Previously reported trends in the LR were confirmed including less discriminatory LRs occurring both for donors and non-donors at low template (for the donor in question) and at high contributor number. We were unable to isolate an effect of allelic sharing. Any apparent effect appears to be largely confounded with increased contributor number.
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