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dc.contributor.authorBakshi, S
dc.contributor.authorMehta, S
dc.contributor.authorKumeria, T
dc.contributor.authorShiddiky, MJA
dc.contributor.authorPopat, A
dc.contributor.authorChoudhury, S
dc.contributor.authorBose, S
dc.contributor.authorNayak, R
dc.date.accessioned2020-12-20T23:51:51Z
dc.date.available2020-12-20T23:51:51Z
dc.date.issued2021
dc.identifier.issn0925-4005
dc.identifier.doi10.1016/j.snb.2020.128803
dc.identifier.urihttp://hdl.handle.net/10072/400423
dc.description.abstractIn recent years, need for rapid, reliable, and low-cost protein biomarker screening has tremendously boosted research into the development of electrochemical immunosensors. In this line, here we report on rapid fabrication and proof-of-concept demonstration of a hyper-branched gold nanostructure (ns@gold) based electrochemical immunosensor for detection of protein biomarker at pg/mL level. We have optimized a simple direct electrochemical process to rapidly generate uniformly distributed high surface area ns@gold within 30 min. These 3D ns@gold show ∼ 8-times enhancement in conductivity and also a rapid surface functionalization via self-assembled monolayers (SAM) of mercaptoundecanoic acid within 45 min. Protein estimation data showed around 94 % immobilization efficiency on the ns@gold surface in comparison to 28.4 % on plain gold surface. A fast SAM formation and quick antibody immobilization steered the assay timing within 4 h. As a proof-of-concept, we used an antibody-antigen interaction for the detection of epithelial growth factor receptor (EGFR) protein. Voltammetric data for the target EGFR receptor resulted a linear working range of 10 pg/mL to 100 ng/mL with a low limit-of-detection of 6.9 pg/mL. High selectivity in detecting target proteins from a pool of non-target proteins using breast cancer cell lines was also demonstrated along with good stability and reusability.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom128803
dc.relation.ispartofjournalSensors and Actuators, B: Chemical
dc.relation.ispartofvolume326
dc.subject.fieldofresearchAtomic, molecular and optical physics
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchMaterials engineering
dc.subject.fieldofresearchcode5102
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode4016
dc.titleRapid fabrication of homogeneously distributed hyper-branched gold nanostructured electrode based electrochemical immunosensor for detection of protein biomarkers
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationBakshi, S; Mehta, S; Kumeria, T; Shiddiky, MJA; Popat, A; Choudhury, S; Bose, S; Nayak, R, Rapid fabrication of homogeneously distributed hyper-branched gold nanostructured electrode based electrochemical immunosensor for detection of protein biomarkers, Sensors and Actuators, B: Chemical, 2021, 326, pp. 128803
dc.date.updated2020-12-18T04:46:22Z
gro.hasfulltextNo Full Text
gro.griffith.authorShiddiky, Muhammad J.


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