dc.contributor.author | Bakshi, S | |
dc.contributor.author | Mehta, S | |
dc.contributor.author | Kumeria, T | |
dc.contributor.author | Shiddiky, MJA | |
dc.contributor.author | Popat, A | |
dc.contributor.author | Choudhury, S | |
dc.contributor.author | Bose, S | |
dc.contributor.author | Nayak, R | |
dc.date.accessioned | 2020-12-20T23:51:51Z | |
dc.date.available | 2020-12-20T23:51:51Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 0925-4005 | |
dc.identifier.doi | 10.1016/j.snb.2020.128803 | |
dc.identifier.uri | http://hdl.handle.net/10072/400423 | |
dc.description.abstract | In recent years, need for rapid, reliable, and low-cost protein biomarker screening has tremendously boosted research into the development of electrochemical immunosensors. In this line, here we report on rapid fabrication and proof-of-concept demonstration of a hyper-branched gold nanostructure (ns@gold) based electrochemical immunosensor for detection of protein biomarker at pg/mL level. We have optimized a simple direct electrochemical process to rapidly generate uniformly distributed high surface area ns@gold within 30 min. These 3D ns@gold show ∼ 8-times enhancement in conductivity and also a rapid surface functionalization via self-assembled monolayers (SAM) of mercaptoundecanoic acid within 45 min. Protein estimation data showed around 94 % immobilization efficiency on the ns@gold surface in comparison to 28.4 % on plain gold surface. A fast SAM formation and quick antibody immobilization steered the assay timing within 4 h. As a proof-of-concept, we used an antibody-antigen interaction for the detection of epithelial growth factor receptor (EGFR) protein. Voltammetric data for the target EGFR receptor resulted a linear working range of 10 pg/mL to 100 ng/mL with a low limit-of-detection of 6.9 pg/mL. High selectivity in detecting target proteins from a pool of non-target proteins using breast cancer cell lines was also demonstrated along with good stability and reusability. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Elsevier | |
dc.relation.ispartofpagefrom | 128803 | |
dc.relation.ispartofjournal | Sensors and Actuators, B: Chemical | |
dc.relation.ispartofvolume | 326 | |
dc.subject.fieldofresearch | Atomic, molecular and optical physics | |
dc.subject.fieldofresearch | Analytical chemistry | |
dc.subject.fieldofresearch | Materials engineering | |
dc.subject.fieldofresearchcode | 5102 | |
dc.subject.fieldofresearchcode | 3401 | |
dc.subject.fieldofresearchcode | 4016 | |
dc.title | Rapid fabrication of homogeneously distributed hyper-branched gold nanostructured electrode based electrochemical immunosensor for detection of protein biomarkers | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dcterms.bibliographicCitation | Bakshi, S; Mehta, S; Kumeria, T; Shiddiky, MJA; Popat, A; Choudhury, S; Bose, S; Nayak, R, Rapid fabrication of homogeneously distributed hyper-branched gold nanostructured electrode based electrochemical immunosensor for detection of protein biomarkers, Sensors and Actuators, B: Chemical, 2021, 326, pp. 128803 | |
dc.date.updated | 2020-12-18T04:46:22Z | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Shiddiky, Muhammad J. | |