Show simple item record

dc.contributor.authorQiao, B
dc.contributor.authorHuang, J
dc.contributor.authorMei, Z
dc.contributor.authorLam, AKY
dc.contributor.authorZhao, J
dc.contributor.authorYing, L
dc.date.accessioned2021-01-04T02:24:36Z
dc.date.available2021-01-04T02:24:36Z
dc.date.issued2020
dc.identifier.issn2234-943X
dc.identifier.doi10.3389/fonc.2020.555757
dc.identifier.urihttp://hdl.handle.net/10072/400590
dc.description.abstractPurpose: The aim is to investigate the impacts of using multiplex immunochemistry (mIHC) staining to analyses the co-expression of programmed death ligand-1 (PD-L1) and tumor infiltrating lymphocytes (TILs) [CD8+ T cells and Forkhead Box Protein 3 (FOXP3)+ regulatory T cells (Tregs)] in different oral diseases, and oral squamous cell carcinoma (OSCC). Methods: Formalin fixed paraffin-embedded tissue sections from different oral diseases were stained with PD-L1 and TILs (CD8+ T cells and FOXP3+ Tregs) by mIHC staining simultaneously. The whole slide was scanned digitally to observe the cell phenotypes stained in the microenvironment. The contents of each slice were read using a computer-aided method to analyze and the cell densities were calculated using statistical software. Results: We were able to characterize the tumor microenvironment (TME) of different oral diseases including oral leukoplakia (OLK), inflammatory gingiva (IG), oral lichen planus (OLP), and squamous cell carcinoma (SCC), with accurate visualization of various immune cells harboring complex immune phenotypes by mIHC staining. The results showed that PD-L1 was up-regulated in SCC tissues at different pathological stages, while CD8 and FOXP3 had no significant changes. The ratio of PD-L1/CD8 was also significantly up-regulated in SCC tissues compared with that of other oral diseases. In advanced stages of OSCC, the FOXP3/CD8 ratio increased, and immunosuppressive TME was more pronounced. In addition, we also found different immune phenotypes: the inflamed phenotype, immune-excluded phenotypes, and immune-desert phenotypes. By locating tumor epithelial cells, we found that PD-L1 expression is in both tumor cells and stromal cells. Conclusions: mIHC is useful for the visualization and evaluation of tumor microenvironment in immuno-oncology research. It allows single-cell imaging in situ and could effectively and quickly determine the immune phenotype of different oral diseases.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherFrontiers Media SA
dc.relation.ispartofpagefrom555757
dc.relation.ispartofjournalFrontiers in Oncology
dc.relation.ispartofvolume10
dc.subject.fieldofresearchOncology and Carcinogenesis
dc.subject.fieldofresearchcode1112
dc.subject.keywordsCD8
dc.subject.keywordsPD-L1
dc.subject.keywordsmultiplex immunochemistry
dc.subject.keywordsoral disease
dc.subject.keywordsoral squamous cell carcinoma
dc.titleAnalysis of Immune Microenvironment by Multiplex Immunohistochemistry Staining in Different Oral Diseases and Oral Squamous Cell Carcinoma
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationQiao, B; Huang, J; Mei, Z; Lam, AKY; Zhao, J; Ying, L, Analysis of Immune Microenvironment by Multiplex Immunohistochemistry Staining in Different Oral Diseases and Oral Squamous Cell Carcinoma, Frontiers in Oncology, 2020, 10, pp. 555757
dcterms.dateAccepted2020-10-19
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2021-01-04T02:12:25Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2020 Qiao, Huang, Mei, Lam, Zhao and Ying. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
gro.hasfulltextFull Text
gro.griffith.authorLam, Alfred K.
gro.griffith.authorQiao, Bin


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record