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  • Key differences between olfactory ensheathing cells and Schwann cells regarding phagocytosis of necrotic cells: implications for transplantation therapies

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    Author(s)
    Nazareth, L
    Shelper, TB
    Chacko, A
    Basu, S
    Delbaz, A
    Lee, JYP
    Chen, M
    St John, JA
    Ekberg, JAK
    Griffith University Author(s)
    St John, James A.
    Ekberg, Jenny A.
    Basu, Souptik
    Chen, Mo
    Delbaz, Ali A.
    Chacko, Anu
    Shelper, Todd B.
    Nazareth, Lynn L.
    Lee, Jia Yu Peppermint
    Year published
    2020
    Metadata
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    Abstract
    Transplantation of peripheral nervous system glia is being explored for treating neural injuries, in particular central nervous system injuries. These glia, olfactory ensheathing cells (OECs) and Schwann cells (SCs), are thought to aid regeneration by clearing necrotic cells, (necrotic bodies, NBs), as well as myelin debris. The mechanism by which the glia phagocytose and traffic NBs are not understood. Here, we show that OECs and SCs recognize phosphatidylserine on NBs, followed by engulfment and trafficking to endosomes and lysosomes. We also showed that both glia can phagocytose and process myelin debris. We compared the ...
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    Transplantation of peripheral nervous system glia is being explored for treating neural injuries, in particular central nervous system injuries. These glia, olfactory ensheathing cells (OECs) and Schwann cells (SCs), are thought to aid regeneration by clearing necrotic cells, (necrotic bodies, NBs), as well as myelin debris. The mechanism by which the glia phagocytose and traffic NBs are not understood. Here, we show that OECs and SCs recognize phosphatidylserine on NBs, followed by engulfment and trafficking to endosomes and lysosomes. We also showed that both glia can phagocytose and process myelin debris. We compared the time-course of glial phagocytosis (of both NBs and myelin) to that of macrophages. Internalization and trafficking were considerably slower in glia than in macrophages, and OECs were more efficient phagocytes than SCs. The two glial types also differed regarding their cytokine responses after NB challenge. SCs produced low amounts of the pro-inflammatory cytokine TNF-α while OECs did not produce detectable TNF-α. Thus, OECs have a higher capacity than SCs for phagocytosis and trafficking, whilst producing lower amounts of pro-inflammatory cytokines. These findings suggest that OEC transplantation into the injured nervous system may lead to better outcomes than SC transplantation.
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    Journal Title
    Scientific Reports
    Volume
    10
    Issue
    1
    DOI
    https://doi.org/10.1038/s41598-020-75850-8
    Copyright Statement
    © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
    Subject
    Biological Sciences
    Science & Technology
    Multidisciplinary Sciences
    Science & Technology - Other Topics
    INFLAMMATORY RESPONSE
    NEURONAL SURVIVAL
    Publication URI
    http://hdl.handle.net/10072/400841
    Collection
    • Journal articles

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