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  • Reliable cell purification and determination of cell purity: crucial aspects of olfactory ensheathing cell transplantation for spinal cord repair

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    Reshamwala426290-Published.pdf (1.669Mb)
    Author(s)
    Reshamwala, Ronak
    Shah, Megha
    Belt, Lucy
    Ekberg, Jenny AK
    St John, James A
    Griffith University Author(s)
    Belt, Lucy A.
    St John, James A.
    Ekberg, Jenny A.
    Shah, Megha
    Reshamwala, Ronak S.
    Year published
    2020
    Metadata
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    Abstract
    Transplantation of olfactory ensheathing cells, the glia of the primary olfactory nervous system, has been trialed for spinal cord injury repair with promising but variable outcomes in animals and humans. Olfactory ensheathing cells can be harvested either from the lamina propria beneath the neuroepithelium in the nasal cavity, or from the olfactory bulb in the brain. As these areas contain several other cell types, isolating and purifying olfactory ensheathing cells is a critical part of the process. It is largely unknown how contaminating cells such as fibroblasts, other glial cell types and supporting cells affect olfactory ...
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    Transplantation of olfactory ensheathing cells, the glia of the primary olfactory nervous system, has been trialed for spinal cord injury repair with promising but variable outcomes in animals and humans. Olfactory ensheathing cells can be harvested either from the lamina propria beneath the neuroepithelium in the nasal cavity, or from the olfactory bulb in the brain. As these areas contain several other cell types, isolating and purifying olfactory ensheathing cells is a critical part of the process. It is largely unknown how contaminating cells such as fibroblasts, other glial cell types and supporting cells affect olfactory ensheathing cell function post-transplantation; these cells may also cause unwanted side-effects. It is also, however, possible that the presence of some of the contaminant cells can improve outcomes. Here, we reviewed the last decade of olfactory ensheathing cell transplantation studies in rodents, with a focus on olfactory ensheathing cell purity. We analyzed how purification methods and resultant cell purity differed between olfactory mucosa- and olfactory bulb-derived cell preparations. We analyzed how the studies reported on olfactory ensheathing cell purity and which criteria were used to define cells as olfactory ensheathing cells. Finally, we analyzed the correlation between cell purity and transplantation outcomes. We found that olfactory bulb-derived olfactory ensheathing cell preparations are typically purer than mucosa-derived preparations. We concluded that there is an association between high olfactory ensheathing cell purity and favourable outcomes, but the lack of olfactory ensheathing cell-specific markers severely hampers the field.
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    Journal Title
    Neural Regeneration Research
    Volume
    15
    Issue
    11
    DOI
    https://doi.org/10.4103/1673-5374.282218
    Copyright Statement
    © The Author(s) 2020. This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License (https://creativecommons.org/licenses/by-nc-sa/3.0/) which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the original work is cited properly.
    Subject
    Biological Sciences
    Neurosciences
    Science & Technology
    Life Sciences & Biomedicine
    Cell Biology
    Neurology
    Publication URI
    http://hdl.handle.net/10072/400842
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    • Journal articles

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