Show simple item record

dc.contributor.authorReshamwala, Ronak
dc.contributor.authorShah, Megha
dc.contributor.authorBelt, Lucy
dc.contributor.authorEkberg, Jenny AK
dc.contributor.authorSt John, James A
dc.date.accessioned2021-01-11T01:10:33Z
dc.date.available2021-01-11T01:10:33Z
dc.date.issued2020
dc.identifier.issn1673-5374
dc.identifier.doi10.4103/1673-5374.282218
dc.identifier.urihttp://hdl.handle.net/10072/400842
dc.description.abstractTransplantation of olfactory ensheathing cells, the glia of the primary olfactory nervous system, has been trialed for spinal cord injury repair with promising but variable outcomes in animals and humans. Olfactory ensheathing cells can be harvested either from the lamina propria beneath the neuroepithelium in the nasal cavity, or from the olfactory bulb in the brain. As these areas contain several other cell types, isolating and purifying olfactory ensheathing cells is a critical part of the process. It is largely unknown how contaminating cells such as fibroblasts, other glial cell types and supporting cells affect olfactory ensheathing cell function post-transplantation; these cells may also cause unwanted side-effects. It is also, however, possible that the presence of some of the contaminant cells can improve outcomes. Here, we reviewed the last decade of olfactory ensheathing cell transplantation studies in rodents, with a focus on olfactory ensheathing cell purity. We analyzed how purification methods and resultant cell purity differed between olfactory mucosa- and olfactory bulb-derived cell preparations. We analyzed how the studies reported on olfactory ensheathing cell purity and which criteria were used to define cells as olfactory ensheathing cells. Finally, we analyzed the correlation between cell purity and transplantation outcomes. We found that olfactory bulb-derived olfactory ensheathing cell preparations are typically purer than mucosa-derived preparations. We concluded that there is an association between high olfactory ensheathing cell purity and favourable outcomes, but the lack of olfactory ensheathing cell-specific markers severely hampers the field.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherMedknow Publications (Wolters Kluwer Health)
dc.relation.ispartofpagefrom2016
dc.relation.ispartofpageto2026
dc.relation.ispartofissue11
dc.relation.ispartofjournalNeural Regeneration Research
dc.relation.ispartofvolume15
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchNeurosciences
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode3209
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsCell Biology
dc.subject.keywordsNeurology
dc.titleReliable cell purification and determination of cell purity: crucial aspects of olfactory ensheathing cell transplantation for spinal cord repair
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationReshamwala, R; Shah, M; Belt, L; Ekberg, JAK; St John, JA, Reliable cell purification and determination of cell purity: crucial aspects of olfactory ensheathing cell transplantation for spinal cord repair, Neural Regeneration Research, 2020, 15 (11), pp. 2016-2026
dcterms.licensehttps://creativecommons.org/licenses/by-nc-sa/3.0/
dc.date.updated2021-01-11T01:05:09Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2020. This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License (https://creativecommons.org/licenses/by-nc-sa/3.0/) which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the original work is cited properly.
gro.hasfulltextFull Text
gro.griffith.authorSt John, James A.
gro.griffith.authorEkberg, Jenny A.
gro.griffith.authorShah, Megha
gro.griffith.authorReshamwala, Ronak S.


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record