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  • The properties and contribution of the Corynebacterium glutamicum MscS variant to fine-tuning of osmotic adaptation

    Author(s)
    Boerngen, Kirsten
    Battle, Andrew R
    Moeker, Nina
    Morbach, Susanne
    Marin, Kay
    Martinac, Boris
    Kraemer, Reinhard
    Griffith University Author(s)
    Battle, Andrew
    Year published
    2010
    Metadata
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    Abstract
    Based on sequence similarity, the mscCG gene product of Corynebacterium glutamicum belongs to the family of MscS-type mechanosensitive channels. In order to investigate the physiological significance of MscCG in response to osmotic shifts in detail, we studied its properties using both patch-clamp techniques and betaine efflux kinetics. After heterologous expression in an Escherichia coli strain devoid of mechanosensitive channels, in patch-clamp analysis of giant E. coli spheroplasts MscCG showed the typical pressure dependent gating behavior of a stretch-activated channel with a current/voltage dependence indicating ...
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    Based on sequence similarity, the mscCG gene product of Corynebacterium glutamicum belongs to the family of MscS-type mechanosensitive channels. In order to investigate the physiological significance of MscCG in response to osmotic shifts in detail, we studied its properties using both patch-clamp techniques and betaine efflux kinetics. After heterologous expression in an Escherichia coli strain devoid of mechanosensitive channels, in patch-clamp analysis of giant E. coli spheroplasts MscCG showed the typical pressure dependent gating behavior of a stretch-activated channel with a current/voltage dependence indicating a strongly rectifying behavior. Apart from that, MscCG is characterized by significant functional differences with respect to conductance, ion selectivity and desensitation behavior as compared to MscS from E. coli. Deletion and complementation studies in C. glutamicum showed a significant contribution of MscCG to betaine efflux in response to hypoosmotic conditions. A detailed analysis of concomitant betaine uptake (by the betaine transporter BetP) and efflux (by MscCG) under hyperosmotic conditions indicates that MscCG may act in osmoregulation in C. glutamicum by fine-tuning the steady state concentration of compatible solutes in the cytoplasm which are accumulated in response to hyperosmotic stress.
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    Journal Title
    Biochimica et Biophysica Acta (BBA) - Biomembranes
    Volume
    1798
    Issue
    11
    DOI
    https://doi.org/10.1016/j.bbamem.2010.06.022
    Subject
    Biochemistry and cell biology
    Structural biology (incl. macromolecular modelling)
    Other biological sciences
    Chemical engineering
    Publication URI
    http://hdl.handle.net/10072/40107
    Collection
    • Journal articles

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