Show simple item record

dc.contributor.authorYou, Hong
dc.contributor.authorHarvie, Marina
dc.contributor.authorDu, Xiaofeng
dc.contributor.authorRivera, Vanessa
dc.contributor.authorZhang, Ping
dc.contributor.authorMcManus, Donald P
dc.date.accessioned2021-01-15T04:35:13Z
dc.date.available2021-01-15T04:35:13Z
dc.date.issued2018
dc.identifier.issn1422-0067
dc.identifier.doi10.3390/ijms19103088
dc.identifier.urihttp://hdl.handle.net/10072/401154
dc.description.abstractThere is a pressing need to develop vaccines for schistosomiasis given the current heavy dependency on praziquantel as the only available drug for treatment. We previously showed the ligand domain of the Schistosoma japonicum insulin receptor 1 and 2 (rSjLD1 and 2) fusion proteins conferred solid protection in mice against challenge infection with S. japonicum. To improve vaccine efficacy, we compared the immunogenicity and protective efficacy of rSjLD1 on its own and in combination with S. japonicum triose-phosphate isomerase (SjTPI), formulated with either of two adjuvants (QuilA and montanide ISA 720VG) in murine vaccine trials against S. japonicum challenge. The level of protection was higher in mice vaccinated only with rSjLD1 formulated with either adjuvant; rSjTPI or the rSjTPI-rSjLD1 combination resulted in a lower level of protection. Mirroring our previous results, there were significant reductions in the number of female worms (30⁻44%), faecal eggs (61⁻68%), liver eggs (44⁻56%), intestinal eggs (46⁻48%) and mature intestinal eggs (58⁻63%) in the rSjLD1-vaccinated mice compared with the adjuvant only groups. At 6-weeks post-cercarial challenge, a significantly increased production of interferon gamma (IFNγ) in rSjLD1-stimulated splenic CD4⁺ T cells was observed in the rSjLD1-vaccinated mice suggesting a Th1-type response is associated with the generated level of protective efficacy.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherMDPI AG
dc.relation.ispartofpagefrom3088
dc.relation.ispartofpageto3088
dc.relation.ispartofissue10
dc.relation.ispartofjournalInternational Journal of Molecular Sciences
dc.relation.ispartofvolume19
dc.subject.fieldofresearchOther Chemical Sciences
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchOther Biological Sciences
dc.subject.fieldofresearchcode0399
dc.subject.fieldofresearchcode0604
dc.subject.fieldofresearchcode0699
dc.subject.keywordsSchistosoma japonicum
dc.subject.keywordsinsulin receptor
dc.subject.keywordsmurine model
dc.subject.keywordstriose-phosphate isomerase
dc.subject.keywordsvaccine
dc.titleProtective Immune Responses Generated in a Murine Model Following Immunization with Recombinant Schistosoma japonicum Insulin Receptor
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationYou, H; Harvie, M; Du, X; Rivera, V; Zhang, P; McManus, DP, Protective Immune Responses Generated in a Murine Model Following Immunization with Recombinant Schistosoma japonicum Insulin Receptor, International Journal of Molecular Sciences, 2018, 19 (10), pp. 3088-3088
dcterms.dateAccepted2018-10-05
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2021-01-15T04:33:23Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorZhang, Ping


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record