dc.contributor.author | Hsu, Hung-Te | |
dc.contributor.author | Tseng, Yu-Ting | |
dc.contributor.author | Lo, Yi-Ching | |
dc.contributor.author | Wu, Sheng-Nan | |
dc.date.accessioned | 2021-01-15T05:21:49Z | |
dc.date.available | 2021-01-15T05:21:49Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1471-2202 | en_US |
dc.identifier.doi | 10.1186/s12868-014-0135-1 | en_US |
dc.identifier.uri | http://hdl.handle.net/10072/401161 | |
dc.description.abstract | Background: Naringenin (NGEN) is a citrus bioflavonoid known to have beneficial health properties; however, the ionic mechanism of its actions remains largely unclear. In this study, we attempted to evaluate the possible effects of NGEN on K+ currents in NSC-34 neuronal cells and in HEK293T cells expressing α-hSlo. Results: NGEN increased M-type K+ current (IK(M)) in a concentration-dependent manner with an EC50 value of 9.8 μM in NSC-34 cells. NGEN shifted the activation curve of IK(M) conductance to the more negative potentials. In cell-attached recordings, NGEN or flupirtine enhanced the activity of M-type K+ (KM) channels with no changes in single-channel amplitude. NGEN (10 μM) had minimal effect on erg-mediated K+ currents. Under cell-attached voltage-clamp recordings, NGEN decreased the frequency of spontaneous action currents and further application of linopirdine can reverse NGEN-induced inhibition of firing. In HEK293T cells expressing α-hSlo, this compound increased the amplitude of Ca2+-activated K+ current (IK(Ca)). Under inside-out recordings, NGEN applied to the intracellular side of the detached patch enhanced the activity of large-conductance Ca2+-activated K+ (BKCa) channels. Moreover, from the study of a modeled neuron, burst firing of simulated action potentials (APs) was reduced in the presence of the increased conductances of both KM and KCa channels. Fast-slow analysis of AP bursting from this model also revealed that as the conductances of both KM and BKCa channels were increased by two-fold, the voltage nullcline was shifted in an upward direction accompanied by the compression of burst trajectory. Conclusions: The present results demonstrate that activation of both KM and BKCa channels caused by NGEN might combine to influence neuronal activity if similar channels were functionally co-expressed in central neurons in vivo. | en_US |
dc.description.peerreviewed | Yes | en_US |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Springer Science and Business Media LLC | en_US |
dc.relation.ispartofpagefrom | 135 | en_US |
dc.relation.ispartofissue | 1 | en_US |
dc.relation.ispartofjournal | BMC Neuroscience | en_US |
dc.relation.ispartofvolume | 15 | en_US |
dc.subject.fieldofresearch | Biochemistry and Cell Biology | en_US |
dc.subject.fieldofresearch | Neurosciences | en_US |
dc.subject.fieldofresearch | Cognitive Sciences | en_US |
dc.subject.fieldofresearchcode | 0601 | en_US |
dc.subject.fieldofresearchcode | 1109 | en_US |
dc.subject.fieldofresearchcode | 1702 | en_US |
dc.title | Ability of naringenin, a bioflavonoid, to activate M-type potassium current in motor neuron-like cells and to increase BKCa-channel activity in HEK293T cells transfected with α-hSlo subunit | en_US |
dc.type | Journal article | en_US |
dc.type.description | C1 - Articles | en_US |
dcterms.bibliographicCitation | Hsu, H-T; Tseng, Y-T; Lo, Y-C; Wu, S-N, Ability of naringenin, a bioflavonoid, to activate M-type potassium current in motor neuron-like cells and to increase BKCa-channel activity in HEK293T cells transfected with α-hSlo subunit, BMC Neuroscience, 2014, 15 (1), pp. 135 | en_US |
dcterms.license | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.date.updated | 2021-01-15T05:18:49Z | |
dc.description.version | Version of Record (VoR) | en_US |
gro.rights.copyright | © 2015 Hsu et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | en_US |
gro.hasfulltext | Full Text | |
gro.griffith.author | Tseng, Tammy | |