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dc.contributor.authorGrau, Marijke
dc.contributor.authorKuck, Lennart
dc.contributor.authorDietz, Thomas
dc.contributor.authorBloch, Wilhelm
dc.contributor.authorSimmonds, Michael J
dc.date.accessioned2021-01-19T00:46:50Z
dc.date.available2021-01-19T00:46:50Z
dc.date.issued2021
dc.identifier.issn2079-7737
dc.identifier.doi10.3390/biology10010047
dc.identifier.urihttp://hdl.handle.net/10072/401270
dc.description.abstractRed blood cell (RBC) deformability is an essential component of microcirculatory function that appears to be enhanced by physiological shear stress, while being negatively affected by supraphysiological shears and/or free radical exposure. Given that blood contains RBCs with non-uniform physical properties, whether all cells equivalently tolerate mechanical and oxidative stresses remains poorly understood. We thus partitioned blood into old and young RBCs which were exposed to phenazine methosulfate (PMS) that generates intracellular superoxide and/or specific mechanical stress. Measured RBC deformability was lower in old compared to young RBCs. PMS increased total free radicals in both sub-populations, and RBC deformability decreased accordingly. Shear exposure did not affect reactive species in the sub-populations but reduced RBC nitric oxide synthase (NOS) activation and intriguingly increased RBC deformability in old RBCs. The co-application of PMS and shear exposure also improved cellular deformability in older cells previously exposed to reactive oxygen species (ROS), but not in younger cells. Outputs of NO generation appeared dependent on cell age; in general, stressors applied to younger RBCs tended to induce S-nitrosylation of RBC cytoskeletal proteins, while older RBCs tended to reflect markers of nitrosative stress. We thus present novel findings pertaining to the interplay of mechanical stress and redox metabolism in circulating RBC sub-populations.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherMDPI AG
dc.relation.ispartofpagefrom47
dc.relation.ispartofissue1
dc.relation.ispartofjournalBiology
dc.relation.ispartofvolume10
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchcode06
dc.subject.keywordsKeywords: blood rheology
dc.subject.keywordsnitric oxide
dc.subject.keywordsoxidative stress
dc.subject.keywordsred blood cell deformability
dc.subject.keywordsshear stress conditioning
dc.titleSub-Fractions of Red Blood Cells Respond Differently to Shear Exposure Following Superoxide Treatment
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationGrau, M; Kuck, L; Dietz, T; Bloch, W; Simmonds, MJ, Sub-Fractions of Red Blood Cells Respond Differently to Shear Exposure Following Superoxide Treatment., Biology, 2021, 10 (1), pp. 47
dcterms.dateAccepted2021-01-07
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2021-01-19T00:36:52Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorSimmonds, Michael J.
gro.griffith.authorKuck, Lennart L.


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