dc.contributor.author | Dhanani, JA | |
dc.contributor.author | Goodman, S | |
dc.contributor.author | Ahern, B | |
dc.contributor.author | Cohen, J | |
dc.contributor.author | Fraser, JF | |
dc.contributor.author | Barnett, A | |
dc.contributor.author | Diab, S | |
dc.contributor.author | Bhatt, M | |
dc.contributor.author | Roberts, JA | |
dc.date.accessioned | 2021-01-28T22:28:22Z | |
dc.date.available | 2021-01-28T22:28:22Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 0924-8579 | |
dc.identifier.doi | 10.1016/j.ijantimicag.2020.106232 | |
dc.identifier.uri | http://hdl.handle.net/10072/401513 | |
dc.description.abstract | Background: Ventilator-associated pneumonia is common and is treated using nebulized antibiotics. Although adequate pulmonary biodistribution is important for antibiotic effect, there is a lack of data for both intravenous (IV) and nebulized antibiotic administration during mechanical ventilation. Objective: To describe the comparative pulmonary regional distribution of IV and nebulized technetium-99m-labeled tobramycin (99mTc-tobramycin) 400 mg in a mechanically-ventilated ovine model. Methods: The study was performed in a mechanically-ventilated ovine model. 99mTc-tobramycin 400 mg was obtained using a radiolabeling process. Computed tomography (CT) was performed. Ten sheep were given 99mTc-tobramycin 400 mg via either an IV (five sheep) or nebulized (five sheep) route. Planar images (dorsal, ventral, left lateral and right lateral) were obtained using a gamma camera. Blood samples were obtained every 15 min for 1 h (4 time points) and lung, liver, both kidney, and urine samples were obtained post-mortem. Results: Ten sheep were anesthetized and mechanically ventilated. Whole-lung deposition of nebulized 99mTc-tobramycin 400 mg was significantly lower than with IV (8.8% vs. 57.1%, P<0.001). For both administration routes, there was significantly lower deposition in upper lung zones compared with the rest of the lungs. Dorsal deposition was significantly higher with nebulized 99mTc-tobramycin 400 mg compared with IV (68.9% vs. 58.9%, P=0.003). Lung concentrations of 99mTc-tobramycin were higher with IV compared with nebulized administration. There were significantly higher concentrations of 99mTc-tobramycin in blood, liver and urine with IV administration compared with nebulized. Conclusions: Nebulization resulted in lower whole and regional lung deposition of 99mTc-tobramycin compared with IV administration and appeared to be associated with low blood and extra-pulmonary organ concentrations. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Elsevier BV | |
dc.relation.ispartofpagefrom | 106232 | |
dc.relation.ispartofissue | 2 | |
dc.relation.ispartofjournal | International Journal of Antimicrobial Agents | |
dc.relation.ispartofvolume | 57 | |
dc.subject.fieldofresearch | Medical microbiology | |
dc.subject.fieldofresearch | Pharmacology and pharmaceutical sciences | |
dc.subject.fieldofresearchcode | 3207 | |
dc.subject.fieldofresearchcode | 3214 | |
dc.subject.keywords | (99m)Tc-tobramycin | |
dc.subject.keywords | nebulized antibiotics | |
dc.subject.keywords | planar scintigraphy | |
dc.subject.keywords | pulmonary biodistribution | |
dc.subject.keywords | tissue quantification | |
dc.title | Comparative lung distribution of radiolabeled tobramycin between nebulized and intravenous administration in a mechanically-ventilated ovine model, an observational study | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dcterms.bibliographicCitation | Dhanani, JA; Goodman, S; Ahern, B; Cohen, J; Fraser, JF; Barnett, A; Diab, S; Bhatt, M; Roberts, JA, Comparative lung distribution of radiolabeled tobramycin between nebulized and intravenous administration in a mechanically-ventilated ovine model, an observational study, International Journal of Antimicrobial Agents, 2020, 57 (2), pp. 106232 | |
dcterms.dateAccepted | 2020-11-14 | |
dc.date.updated | 2021-01-28T22:00:06Z | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Fraser, John F. | |