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dc.contributor.authorVanova, Katerina
dc.contributor.authorKrobova, Linda
dc.contributor.authorBezawork-Geleta, Ayenachem
dc.contributor.authorBoukalova, Stepana
dc.contributor.authorPacak, Karel
dc.contributor.authorGhayee, Hans Kumar
dc.contributor.authorRohlena, Jakub
dc.contributor.authorNeuzil, Jiri
dc.date.accessioned2021-01-29T01:02:03Z
dc.date.available2021-01-29T01:02:03Z
dc.date.issued2019
dc.identifier.issn0014-2972
dc.identifier.urihttp://hdl.handle.net/10072/401531
dc.description.abstractPheochromocytoma and paraganglioma (PHEO/PGL) are neuroendocrine tumours that are frequently associated with mutations in mitochondrial complex II (CII) subunits SDHA‐D. Clinical data indicate that SDHB‐deficient PHEO/PGL are less proliferative but are associated with higher invasiveness and metastasis. We have previously shown that both the loss of SDHB and/or depletion of mitochondrial (mt)DNA in the breast cancer cell line MDA‐MB‐231 leads to alternative assembly of CIIlow, which maintains homeostatic control of metabolite synthesis under bioenergetics stress and supports tumour growth. However, PHEO/PGL malignancies arise from the chromaffin tissue; it is therefore expected that the epithelial‐derived cells, such as breast cancer cells, are not an optimal model to decipher the consequences of CII mutations in PHEO/PGL. For this reason, human chromaffin‐derived pheochromocytoma cancer cells line hPheo1 was used to introduce SDHB deficiency (SDHB KO cells) by gene manipulation. PGL tissue, mitochondria, and whole cell lysate were used for respiratory assay, native blue gel electrophoresis (NBGE) and western blotting. Similar to patient‐derived SDHB‐deficient paraganglioma tissue, SDHB KO cells featured substantial CII low in the absence of fully assembled CII on NBGE, accompanied by prominent decrease of respiration and other relevant alterations. Further analysis of this unique model is ongoing. Our data provide a new insight into pathological features of hard‐to‐treat SDHx‐related PHEO/PGL. We suggest that the existence of CIIlow in patients with SDHB‐mutated paraganglioma substantially contributes to tumorigenicity, and interference with CIIlow could ameliorate severe pathological outcomes, including enhanced migration and invasiveness.
dc.languageEnglish
dc.publisherWiley
dc.publisher.urihttps://onlinelibrary.wiley.com/doi/10.1111/eci.13109
dc.relation.ispartofconferencenamethe 53rd Annual Scientific Meeting of the European Society for Clinical Investigation
dc.relation.ispartofconferencetitleEuropean Journal of Clinical Investigation
dc.relation.ispartofdatefrom2019-05-22
dc.relation.ispartofdateto2019-05-24
dc.relation.ispartoflocationCoimbra, Portugal
dc.relation.ispartofpagefrom193
dc.relation.ispartofpageto193
dc.relation.ispartofissueS1
dc.relation.ispartofvolume49
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchcode3202
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsMedicine, General & Internal
dc.subject.keywordsMedicine, Research & Experimental
dc.subject.keywordsGeneral & Internal Medicine
dc.titleHuman pheochromocytoma cancer cells as a plausible model of mitochondrial complex II-linked tumorigenesis
dc.typeConference output
dc.type.descriptionE3 - Conferences (Extract Paper)
dcterms.bibliographicCitationVanova, K; Krobova, L; Bezawork-Geleta, A; Boukalova, S; Pacak, K; Ghayee, HK; Rohlena, J; Neuzil, J, Human pheochromocytoma cancer cells as a plausible model of mitochondrial complex II-linked tumorigenesis, European Journal of Clinical Investigation, 2019, 49 (S1), pp. 193-193
dc.date.updated2021-01-29T00:58:15Z
gro.hasfulltextNo Full Text
gro.griffith.authorNeuzil, Jiri


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