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dc.contributor.authorM. Hashimi, Saeeden_US
dc.contributor.authorG. Birch, Roberten_US
dc.date.accessioned2017-05-03T11:49:54Z
dc.date.available2017-05-03T11:49:54Z
dc.date.issued2010en_US
dc.date.modified2011-08-19T06:46:11Z
dc.identifier.issn0175-7598en_US
dc.identifier.doi10.1007/s00253-010-2620-5en_AU
dc.identifier.urihttp://hdl.handle.net/10072/40182
dc.description.abstractAlbicidins are potent DNA-gyrase-inhibiting antibiotics and phytotoxins synthesised by Xanthomonas albilineans. Functions have been deduced for some clustered biosynthetic genes, including a PKS-NRPS megasynthase, methyltransferases and regulatory genes, and resistance genes including a transporter and a gyrase-binding protein. More puzzling is the presence in this cluster of apparent aromatic metabolism genes. Here, we describe functional analysis of several such genes and propose a model for their role. An apparent benzoate CoA ligase (xabE) proved essential for albicidin production and pathogenicity. A neighbouring operon includes genes for p-aminobenzoate (PABA) metabolism. A PABA synthase fusion (pabAB) restored prototrophy in pabA and pabB mutants of Escherichia coli, proving functionality. Inactivation of pabAB increased susceptibility to sulphanilamide but did not block albicidin production. X. albilineans contains a remote pabB gene which evidently supplies enough PABA for albicidin biosynthesis in culture. Additional capacity from pabAB may be advantageous in more demanding environments such as infected plants. Downstream from pabAB are a known resistance gene (albG) and ubiC which encodes a p-hydroxybenzoate (PHBA) synthase. PHBA protects X. albilineans from inhibition by PABA. Therefore, coordinated expression may protect X. albilineans against toxicity of both the PABA intermediate and the albicidin product, under conditions that induce high-level antibiotic biosynthesis.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherSpringeren_US
dc.publisher.placeGermanyen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom1475en_US
dc.relation.ispartofpageto1485en_US
dc.relation.ispartofissue4en_US
dc.relation.ispartofjournalApplied Microbiology and Biotechnologyen_US
dc.relation.ispartofvolume87en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchTechnology not elsewhere classifieden_US
dc.subject.fieldofresearchcode109999en_US
dc.titleFunctional analysis of genes for benzoate metabolism in the albicidin biosynthetic region of Xanthomonas albilineansen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2010
gro.hasfulltextNo Full Text


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