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dc.contributor.authorHashimi, Saeed M
dc.contributor.authorBirch, Robert G
dc.date.accessioned2017-05-03T11:49:54Z
dc.date.available2017-05-03T11:49:54Z
dc.date.issued2010
dc.date.modified2011-08-19T06:46:11Z
dc.identifier.issn0175-7598
dc.identifier.doi10.1007/s00253-010-2620-5
dc.identifier.urihttp://hdl.handle.net/10072/40182
dc.description.abstractAlbicidins are potent DNA-gyrase-inhibiting antibiotics and phytotoxins synthesised by Xanthomonas albilineans. Functions have been deduced for some clustered biosynthetic genes, including a PKS-NRPS megasynthase, methyltransferases and regulatory genes, and resistance genes including a transporter and a gyrase-binding protein. More puzzling is the presence in this cluster of apparent aromatic metabolism genes. Here, we describe functional analysis of several such genes and propose a model for their role. An apparent benzoate CoA ligase (xabE) proved essential for albicidin production and pathogenicity. A neighbouring operon includes genes for p-aminobenzoate (PABA) metabolism. A PABA synthase fusion (pabAB) restored prototrophy in pabA and pabB mutants of Escherichia coli, proving functionality. Inactivation of pabAB increased susceptibility to sulphanilamide but did not block albicidin production. X. albilineans contains a remote pabB gene which evidently supplies enough PABA for albicidin biosynthesis in culture. Additional capacity from pabAB may be advantageous in more demanding environments such as infected plants. Downstream from pabAB are a known resistance gene (albG) and ubiC which encodes a p-hydroxybenzoate (PHBA) synthase. PHBA protects X. albilineans from inhibition by PABA. Therefore, coordinated expression may protect X. albilineans against toxicity of both the PABA intermediate and the albicidin product, under conditions that induce high-level antibiotic biosynthesis.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoen_AU
dc.publisherSpringer
dc.publisher.placeGermany
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1475
dc.relation.ispartofpageto1485
dc.relation.ispartofissue4
dc.relation.ispartofjournalApplied Microbiology and Biotechnology
dc.relation.ispartofvolume87
dc.rights.retentionY
dc.subject.fieldofresearchTechnology not elsewhere classified
dc.subject.fieldofresearchcode109999
dc.titleFunctional analysis of genes for benzoate metabolism in the albicidin biosynthetic region of Xanthomonas albilineans
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2010
gro.hasfulltextNo Full Text
gro.griffith.authorHashimi, Saeed M.


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