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  • Combining analytical and in vitro techniques for comprehensive assessments of chemical exposure and effect in green sea turtles (Chelonia mydas)

    Author(s)
    Finlayson, KA
    Leusch, FDL
    Villa, CA
    Limpus, CJ
    van de Merwe, JP
    Griffith University Author(s)
    Leusch, Frederic
    van de Merwe, Jason P.
    Finlayson, Kimberly A.
    Year published
    2021
    Metadata
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    Abstract
    Sea turtle populations foraging in coastal areas adjacent to human activity can be exposed to numerous chemical contaminants for long periods of time. For trace elements, well-developed, sensitive and inexpensive analytical techniques remain the most effective method for assessing exposure in sea turtles. However, there are many thousands more organic contaminants present in sea turtles, often at low levels as complex mixtures. Recently developed species-specific in vitro bioassays provide an effective means to identify the presence, and effect of, organic chemicals in sea turtles. This study used a combination of chemical ...
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    Sea turtle populations foraging in coastal areas adjacent to human activity can be exposed to numerous chemical contaminants for long periods of time. For trace elements, well-developed, sensitive and inexpensive analytical techniques remain the most effective method for assessing exposure in sea turtles. However, there are many thousands more organic contaminants present in sea turtles, often at low levels as complex mixtures. Recently developed species-specific in vitro bioassays provide an effective means to identify the presence, and effect of, organic chemicals in sea turtles. This study used a combination of chemical analysis and effects-based bioassays to provide complementary information on chemical exposure and effects for three green turtle foraging populations (Chelonia mydas) in southern Queensland, Australia. Blood was collected from foraging sub-adult green turtles captured in Moreton Bay, Hervey Bay, and Port Curtis. Twenty-six trace elements were measured in whole blood using ICP-MS. Organic contaminants in turtle blood were extracted via QuEChERS and applied to primary green turtle skin fibroblast cell in vitro assays for two toxicity endpoints; cytotoxicity and oxidative stress. The trace element analysis and bioassay results indicated site-specific differences between foraging populations. In particular, turtles from Moreton Bay, a heavily populated coastal embayment, had pronounced cytotoxicity and oxidative stress from organic blood extracts, and elevated concentrations of Cs, Ag, and Zn relative to the other sites. Incorporating traditional chemical analysis with novel effects-based methods can provide a comprehensive assessment of chemical risk in sea turtle populations, contributing to the conservation and management of these threatened species.
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    Journal Title
    Chemosphere
    Volume
    274
    DOI
    https://doi.org/10.1016/j.chemosphere.2021.129752
    Subject
    Biological Oceanography
    Marine and Estuarine Ecology (incl. Marine Ichthyology)
    Publication URI
    http://hdl.handle.net/10072/401855
    Collection
    • Journal articles

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