Mir-3928 in Saliva as Potential Biomarker for Head and Neck Squamous Cell Carcinoma
Author(s)
Fadhil, Rushdi
Wei, Ming
Nikolarakos, Dimitrios
Good, David
Nair, Raj
Griffith University Author(s)
Year published
2019
Metadata
Show full item recordAbstract
Objectives: To determine whether salivary miR-3928 can be used for early head and neck cancer diagnostic purpose.
Methods: The expression level of human salivary miR-3928 was investigated in 230 supernatant saliva samples. These samples were collected from head and neck squamous cell carcinoma (HNSCC) patients (n = 150) and healthy individuals (n = 80). Quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to detect the expression level of the miRNA.
Results: Total RNA was isolated in average 160 ng/µl from 500 µl of supernatant saliva. Overall, miR-3928 expression was revealed to be correlated ...
View more >Objectives: To determine whether salivary miR-3928 can be used for early head and neck cancer diagnostic purpose. Methods: The expression level of human salivary miR-3928 was investigated in 230 supernatant saliva samples. These samples were collected from head and neck squamous cell carcinoma (HNSCC) patients (n = 150) and healthy individuals (n = 80). Quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to detect the expression level of the miRNA. Results: Total RNA was isolated in average 160 ng/µl from 500 µl of supernatant saliva. Overall, miR-3928 expression was revealed to be correlated with HNC. We found that salivary miR-3928 was significantly down regulated in supernatant saliva of HNC patients (P< 0.05). Conclusions: This study reported that down-regulate of miR-3928 could be used as salivary biomarker for HNSCC detection.
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View more >Objectives: To determine whether salivary miR-3928 can be used for early head and neck cancer diagnostic purpose. Methods: The expression level of human salivary miR-3928 was investigated in 230 supernatant saliva samples. These samples were collected from head and neck squamous cell carcinoma (HNSCC) patients (n = 150) and healthy individuals (n = 80). Quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to detect the expression level of the miRNA. Results: Total RNA was isolated in average 160 ng/µl from 500 µl of supernatant saliva. Overall, miR-3928 expression was revealed to be correlated with HNC. We found that salivary miR-3928 was significantly down regulated in supernatant saliva of HNC patients (P< 0.05). Conclusions: This study reported that down-regulate of miR-3928 could be used as salivary biomarker for HNSCC detection.
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Conference Title
97th International Association of Dental Research (IADR) General Session
Subject
Oncology and carcinogenesis