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  • Lamp based detection of sars-cov-2 with low-cost off-the-shelf components

    Author(s)
    Sreejith, KR
    Umer, M
    Soda, N
    Kasetsirikul, S
    Shiddiky, MJA
    Nguyen, NT
    Griffith University Author(s)
    Shiddiky, Muhammad J.
    Soda, Narshone
    Nguyen, Nam-Trung
    Umer, Muhammad
    Kasetsirikul, Surasak
    Year published
    2020
    Metadata
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    Abstract
    This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for rapid SARS-CoV-2 detection. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 minutes using clinically relevant starting concentrations of the synthetic template.This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for rapid SARS-CoV-2 detection. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 minutes using clinically relevant starting concentrations of the synthetic template.
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    Conference Title
    MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    Publisher URI
    https://microtas2020.org/
    Subject
    Classical physics
    Nanotechnology
    Publication URI
    http://hdl.handle.net/10072/401965
    Collection
    • Conference outputs

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