Lamp based detection of sars-cov-2 with low-cost off-the-shelf components
Author(s)
Sreejith, KR
Umer, M
Soda, N
Kasetsirikul, S
Shiddiky, MJA
Nguyen, NT
Griffith University Author(s)
Year published
2020
Metadata
Show full item recordAbstract
This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for rapid SARS-CoV-2 detection. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 minutes using clinically relevant starting concentrations of the synthetic template.This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for rapid SARS-CoV-2 detection. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 minutes using clinically relevant starting concentrations of the synthetic template.
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Conference Title
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher URI
Subject
Classical physics
Nanotechnology