dc.contributor.author | Farhana, FZ | |
dc.contributor.author | Umer, M | |
dc.contributor.author | Saeed, A | |
dc.contributor.author | Pannu, AS | |
dc.contributor.author | Shahbazi, M | |
dc.contributor.author | Jabur, A | |
dc.contributor.author | Nam, HJ | |
dc.contributor.author | Ostrikov, K | |
dc.contributor.author | Sonar, P | |
dc.contributor.author | Firoz, SH | |
dc.contributor.author | Shiddiky, MJA | |
dc.date.accessioned | 2021-02-11T02:02:47Z | |
dc.date.available | 2021-02-11T02:02:47Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 2574-0970 | |
dc.identifier.doi | 10.1021/acsanm.0c02807 | |
dc.identifier.uri | http://hdl.handle.net/10072/401977 | |
dc.description.abstract | Magnetic nanozymes with peroxidase-mimicking activity have been widely investigated for developing molecular biosensors. Herein, we report a starch-assisted method for the synthesis of a novel class of carboxyl group-functionalized iron oxide nanoparticles (C-IONPs). Scanning electron and transmission electron microscopy analysis revealed that the nanoparticles possess a spherical shape with an average size of ∼250 nm. Peroxidase-mimicking activity of C-IONPs was investigated through catalytic oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2. The results showed that nanoparticles follow typical Michaelis−Menten kinetics and exhibit excellent affinity toward TMB and H2O2 with estimated KM and VMax values of 0.0992 mM and 0.156 × 10−8 Ms−1 for TMB and 114 mM and 0.197 × 10−8 Ms−1 for H2O2, respectively. C-IONPs were used to develop a simple method for the direct isolation and quantification of disease-specific exosomes. This method utilized a two-step strategy that involved (a) initial isolation of bulk exosomes present in the sample media using tetraspanin biomarker (i.e., CD9)-functionalized C-IONPs and (b) subsequent electrochemical quantification of disease-specific exosomes within the captured bulk exosomes using tumor-specific markers (in this case, the ovarian cancer biomarker CA-125). In the first step, C-IONPs were used as “dispersible nanocarriers” to capture the bulk population of exosomes, and in the second step, they were used as nanozymes to generate an enzyme-catalyzed current indicative of the presence of tumor-specific exosomes. Chronoamperometric analysis showed that the method exhibits an excellent specificity for OVCAR3 cell-derived exosomes (linear dynamic range, 6.25 × 105 to 1.0 × 107 exosomes/mL; detection limit, 1.25 × 106 exosomes/mL) with a relative standard deviation of <5.0% (n = 3). Due to their excellent enzyme catalytic activity, ability to magnetically separate the target from bulk samples, and versatile bioconjugation capability (because of the −COOH group), C-IONPs are a promising candidate for the development of advanced exosome biosensing assays for various clinical applications. | |
dc.description.peerreviewed | Yes | |
dc.language | en | |
dc.publisher | American Chemical Society (ACS) | |
dc.relation.ispartofjournal | ACS Applied Nano Materials | |
dc.subject.fieldofresearch | Nanotechnology | |
dc.subject.fieldofresearch | Industrial biotechnology | |
dc.subject.fieldofresearch | Macromolecular and materials chemistry | |
dc.subject.fieldofresearchcode | 4018 | |
dc.subject.fieldofresearchcode | 3106 | |
dc.subject.fieldofresearchcode | 3403 | |
dc.title | Isolation and Detection of Exosomes Using Fe2O3 Nanoparticles | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dcterms.bibliographicCitation | Farhana, FZ; Umer, M; Saeed, A; Pannu, AS; Shahbazi, M; Jabur, A; Nam, HJ; Ostrikov, K; Sonar, P; Firoz, SH; Shiddiky, MJA, Isolation and Detection of Exosomes Using Fe2O3 Nanoparticles, ACS Applied Nano Materials, 2021 | |
dc.date.updated | 2021-02-11T02:01:37Z | |
gro.description.notepublic | This publication has been entered as an advanced online version in Griffith Research Online. | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Umer, Muhammad | |
gro.griffith.author | Shiddiky, Muhammad J. | |
gro.griffith.author | Pannu, Amandeep Singh S. | |