Multifunctional graphene micro-islands: Rapid, low-temperature plasma-enabled synthesis and facile integration for bioengineering genosensing applications

View/ Open
File version
Accepted Manuscript (AM)
Author(s)
Pineda, Shafique
Borghi, Fabricio Frizera
Seo, Dong Han
Yick, Samuel
Lawn, Malcolm
van der Laan, Timothy
Han, Zhao Jun
Ostrikov, Kostya Ken
Griffith University Author(s)
Year published
2017
Metadata
Show full item recordAbstract
Here, we present a rapid, low-temperature (200 °C) plasma-enabled synthesis of graphene micro-islands (GMs). Morphological analyses of GMs by scanning electron microscopy (SEM) and atomic force microscopy (AFM) feature a uniform and open-networked array of aggregated graphene sheets. Structural and surface chemical characterizations by Raman spectroscopy and X-ray photoelectron spectroscopy (XPS) support the presence of thin graphitic edges and reactive oxygen functional groups. We demonstrate that these inherent properties of GMs enable its multifunctional capabilities as a bioactive interface. GMs exhibit a biocompatibility ...
View more >Here, we present a rapid, low-temperature (200 °C) plasma-enabled synthesis of graphene micro-islands (GMs). Morphological analyses of GMs by scanning electron microscopy (SEM) and atomic force microscopy (AFM) feature a uniform and open-networked array of aggregated graphene sheets. Structural and surface chemical characterizations by Raman spectroscopy and X-ray photoelectron spectroscopy (XPS) support the presence of thin graphitic edges and reactive oxygen functional groups. We demonstrate that these inherent properties of GMs enable its multifunctional capabilities as a bioactive interface. GMs exhibit a biocompatibility of 80% cell viability with primary fibroblast lung cells after 5 days. Further, GMs were assembled into an impedimetric genosensor, and its performance was characterized by electrochemical impedance spectroscopy (EIS). A dynamic sensing range of 1 pM to 1 nM is reported, and a limit of quantification (LOQ) of 2.03×10−13 M is deduced, with selectivity to single-RNA-base mismatched sequences. The versatile nature of GMs may be explored to enable multi-faceted bioactive platforms for next-generation personalized healthcare technologies.
View less >
View more >Here, we present a rapid, low-temperature (200 °C) plasma-enabled synthesis of graphene micro-islands (GMs). Morphological analyses of GMs by scanning electron microscopy (SEM) and atomic force microscopy (AFM) feature a uniform and open-networked array of aggregated graphene sheets. Structural and surface chemical characterizations by Raman spectroscopy and X-ray photoelectron spectroscopy (XPS) support the presence of thin graphitic edges and reactive oxygen functional groups. We demonstrate that these inherent properties of GMs enable its multifunctional capabilities as a bioactive interface. GMs exhibit a biocompatibility of 80% cell viability with primary fibroblast lung cells after 5 days. Further, GMs were assembled into an impedimetric genosensor, and its performance was characterized by electrochemical impedance spectroscopy (EIS). A dynamic sensing range of 1 pM to 1 nM is reported, and a limit of quantification (LOQ) of 2.03×10−13 M is deduced, with selectivity to single-RNA-base mismatched sequences. The versatile nature of GMs may be explored to enable multi-faceted bioactive platforms for next-generation personalized healthcare technologies.
View less >
Journal Title
Biosensors and Bioelectronics
Volume
89
Issue
Pt 1
Copyright Statement
© 2017 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
Subject
Analytical chemistry
Biomedical engineering
Nanotechnology
Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biophysics
Biotechnology & Applied Microbiology