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dc.contributor.authorAtack, John M
dc.contributor.authorGuo, Chengying
dc.contributor.authorLitfin, Thomas
dc.contributor.authorYang, Long
dc.contributor.authorBlackall, Patrick J
dc.contributor.authorZhou, Yaoqi
dc.contributor.authorJennings, Michael P
dc.date.accessioned2021-02-15T01:42:44Z
dc.date.available2021-02-15T01:42:44Z
dc.date.issued2020
dc.identifier.doi10.1101/2020.06.18.137471
dc.identifier.urihttp://hdl.handle.net/10072/402122
dc.description.abstractN6-Adenine DNA methyltransferases associated with some Type I and Type III restriction-modification (R-M) systems are able to undergo phase variation, randomly switching expression ON or OFF by varying the length of locus-encoded simple sequence repeats (SSRs). This variation of methyltransferase expression results in genome-wide methylation differences and global changes in gene expression. These epigenetic regulatory systems are called phasevarions, phase-variable regulons, and are widespread in bacteria. A distinct switching system has also been described in Type I R-M systems, based on recombination-driven changes in hsdS genes, which dictate the DNA target site. In order to determine the prevalence of recombination-driven phasevarions, we generated a program called RecombinationRepeatSearch to interrogate REBASE and identify the presence and number of inverted repeats of hsdS downstream of Type I R-M loci. We report that 3.9% of Type I R-M systems have duplicated variable hsdS genes containing inverted repeats capable of phase variation. We report the presence of these systems in the major pathogens Enterococcus faecalis and Listeria monocytogenes, which could have important implications for pathogenesis and vaccine development. These data suggest that in addition to SSR-driven phasevarions, many bacteria have independently evolved phase-variable Type I R-M systems via recombination between multiple, variable hsdS genes.
dc.description.peerreviewedYes
dc.publisherAmerican Society of Microbiology
dc.relation.ispartofjournalmSystems
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchcode06
dc.titleSystematic analysis of REBASE identifies numerous Type I restriction-modification systems that contain duplicated, variable hsdS specificity genes that randomly switch methyltransferase specificity by recombination
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationAtack, JM; Guo, C; Litfin, T; Yang, L; Blackall, PJ; Zhou, Y; Jennings, MP, Systematic analysis of REBASE identifies numerous Type I restriction-modification systems that contain duplicated, variable hsdS specificity genes that randomly switch methyltransferase specificity by recombination, mSystems
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2021-02-15T01:31:50Z
dc.description.versionVersion of Record (VoR)
gro.description.notepublicThis publication has been entered as an advanced online version in Griffith Research Online.
gro.rights.copyright© 2020 Atack et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorAtack, John M.
gro.griffith.authorJennings, Michael P.
gro.griffith.authorLitfin, Tom
gro.griffith.authorZhou, Yaoqi


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