dc.contributor.author | Fanning, Jonathon P | |
dc.contributor.author | Obonyo, Nchafatso G | |
dc.contributor.author | Tung, John-Paul | |
dc.contributor.author | Byrne, Liam | |
dc.contributor.author | Simonova, Gabriela | |
dc.contributor.author | Diab, Sara | |
dc.contributor.author | Dunster, Kimble | |
dc.contributor.author | Passmore, Margaret | |
dc.contributor.author | Boon, Ai-Ching | |
dc.contributor.author | Hoe, See Louise | |
dc.contributor.author | Engkilde-Pedersen, Sanne | |
dc.contributor.author | Esguerra-Lallen, Arlanna | |
dc.contributor.author | Fauzi, Hashairi Mohd | |
dc.contributor.author | Anstey, Christopher | |
dc.contributor.author | et al. | |
dc.date.accessioned | 2021-02-16T04:21:01Z | |
dc.date.available | 2021-02-16T04:21:01Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0009-7322 | |
dc.identifier.uri | http://hdl.handle.net/10072/402213 | |
dc.description.abstract | Introduction: Packed red blood cell (PRBC) transfusion in septic shock is generally reserved as a last resort hemodynamic resuscitative measure or for haemoglobin less than 7.0 g/dL. Ex-vivo preservation and storage predispose PRBCs to biochemical / mechanical changes whose impact in a septic host is unknown. This randomised pre-clinical trial specifically evaluates select markers of cardiovascular stress in an ovine model of endotoxemic shock receiving either fresh or stored blood.
Methods: Twenty six anesthetised and mechanically ventilated merino ewes were randomly divided into endotoxemic shock (n = 16) or healthy (n = 10) groups. Endotoxemic shock was induced over 4 hours by infusing an escalating dose (total 11.25 mcg / kg) of lipopolysaccharide, LPS (E.coli 055:B5). During the last hour of LPS infusion, 10 mls / kg of either fresh, ≤ 5 days (n = 8) or stored, ≥ 30 days (n = 8) ovine packed PRBCs were administered. Healthy ewes received either fresh, ≤ 5 days (n = 5) or stored, ≥ 30 days (n = 5) ovine PRBCs at corresponding time points. Invasive haemodynamic monitoring and serial echocardiographic assessments were performed in all. Blood samples were measured for cardiac troponin I (cTnI), atrial natriuretic peptide (ANP) and hyaluronan at baseline (T0), 1, 3, 4, 5, 10 and 16h after which the ewes were euthanized.
Results: Stored versus fresh PRBCs produced raised hyaluronan levels (P < 0.001) from T3 onwards in both sepsis and healthy groups and resulted in significant (P < 0.001) adverse haemodynamic changes (cardiac index, central venous pressure, pulmonary artery pressure and mixed venous oxygen saturation). Elevated ANP was observed between T3 to T10 (P < 0.001) in septic compared to healthy ewes but there was no association with age of PRBCs. No statistical differences were observed between groups for TnI.
Conclusions: Sepsis and storage duration of PRBCs are associated with increased cardiovascular stress suggesting that stored PRBCs may predispose patients with septic shock to poor clinical outcomes. | |
dc.language | English | |
dc.publisher | Lippincott Williams & Wilkins | |
dc.publisher.uri | https://www.ahajournals.org/doi/abs/10.1161/circ.138.suppl_1.17116 | |
dc.relation.ispartofconferencename | American Heart Association's 2018 Scientific Sessions | |
dc.relation.ispartofconferencetitle | Circulation | |
dc.relation.ispartofdatefrom | 2018-11-10 | |
dc.relation.ispartofdateto | 2018-11-14 | |
dc.relation.ispartoflocation | Chicago, Illinois, USA | |
dc.relation.ispartofvolume | 138 | |
dc.subject.fieldofresearch | Cardiovascular medicine and haematology | |
dc.subject.fieldofresearch | Clinical sciences | |
dc.subject.fieldofresearchcode | 3201 | |
dc.subject.fieldofresearchcode | 3202 | |
dc.subject.keywords | Science & Technology | |
dc.subject.keywords | Life Sciences & Biomedicine | |
dc.subject.keywords | Cardiac & Cardiovascular Systems | |
dc.subject.keywords | Peripheral Vascular Disease | |
dc.subject.keywords | Cardiovascular System & Cardiology | |
dc.title | Packed Red Cell Age Associated With Adverse Cardiovascular Changes in an Ovine Model of Septic Shock Resuscitation | |
dc.type | Conference output | |
dc.type.description | E3 - Conferences (Extract Paper) | |
dcterms.bibliographicCitation | Fanning, JP; Obonyo, NG; Tung, J-P; Byrne, L; Simonova, G; Diab, S; Dunster, K; Passmore, M; Boon, A-C; Hoe, SL; Engkilde-Pedersen, S; Esguerra-Lallen, A; Fauzi, HM; Anstey, C; et al., Packed Red Cell Age Associated With Adverse Cardiovascular Changes in an Ovine Model of Septic Shock Resuscitation, Circulation, 2018, 138 | |
dc.date.updated | 2021-02-16T04:12:23Z | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Anstey, Christopher | |