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dc.contributor.authorFanning, Jonathon P
dc.contributor.authorObonyo, Nchafatso G
dc.contributor.authorTung, John-Paul
dc.contributor.authorByrne, Liam
dc.contributor.authorSimonova, Gabriela
dc.contributor.authorDiab, Sara
dc.contributor.authorDunster, Kimble
dc.contributor.authorPassmore, Margaret
dc.contributor.authorBoon, Ai-Ching
dc.contributor.authorHoe, See Louise
dc.contributor.authorEngkilde-Pedersen, Sanne
dc.contributor.authorEsguerra-Lallen, Arlanna
dc.contributor.authorFauzi, Hashairi Mohd
dc.contributor.authorAnstey, Christopher
dc.contributor.authoret al.
dc.date.accessioned2021-02-16T04:21:01Z
dc.date.available2021-02-16T04:21:01Z
dc.date.issued2018
dc.identifier.issn0009-7322
dc.identifier.urihttp://hdl.handle.net/10072/402213
dc.description.abstractIntroduction: Packed red blood cell (PRBC) transfusion in septic shock is generally reserved as a last resort hemodynamic resuscitative measure or for haemoglobin less than 7.0 g/dL. Ex-vivo preservation and storage predispose PRBCs to biochemical / mechanical changes whose impact in a septic host is unknown. This randomised pre-clinical trial specifically evaluates select markers of cardiovascular stress in an ovine model of endotoxemic shock receiving either fresh or stored blood. Methods: Twenty six anesthetised and mechanically ventilated merino ewes were randomly divided into endotoxemic shock (n = 16) or healthy (n = 10) groups. Endotoxemic shock was induced over 4 hours by infusing an escalating dose (total 11.25 mcg / kg) of lipopolysaccharide, LPS (E.coli 055:B5). During the last hour of LPS infusion, 10 mls / kg of either fresh, ≤ 5 days (n = 8) or stored, ≥ 30 days (n = 8) ovine packed PRBCs were administered. Healthy ewes received either fresh, ≤ 5 days (n = 5) or stored, ≥ 30 days (n = 5) ovine PRBCs at corresponding time points. Invasive haemodynamic monitoring and serial echocardiographic assessments were performed in all. Blood samples were measured for cardiac troponin I (cTnI), atrial natriuretic peptide (ANP) and hyaluronan at baseline (T0), 1, 3, 4, 5, 10 and 16h after which the ewes were euthanized. Results: Stored versus fresh PRBCs produced raised hyaluronan levels (P < 0.001) from T3 onwards in both sepsis and healthy groups and resulted in significant (P < 0.001) adverse haemodynamic changes (cardiac index, central venous pressure, pulmonary artery pressure and mixed venous oxygen saturation). Elevated ANP was observed between T3 to T10 (P < 0.001) in septic compared to healthy ewes but there was no association with age of PRBCs. No statistical differences were observed between groups for TnI. Conclusions: Sepsis and storage duration of PRBCs are associated with increased cardiovascular stress suggesting that stored PRBCs may predispose patients with septic shock to poor clinical outcomes.
dc.languageEnglish
dc.publisherLippincott Williams & Wilkins
dc.publisher.urihttps://www.ahajournals.org/doi/abs/10.1161/circ.138.suppl_1.17116
dc.relation.ispartofconferencenameAmerican Heart Association's 2018 Scientific Sessions
dc.relation.ispartofconferencetitleCirculation
dc.relation.ispartofdatefrom2018-11-10
dc.relation.ispartofdateto2018-11-14
dc.relation.ispartoflocationChicago, Illinois, USA
dc.relation.ispartofvolume138
dc.subject.fieldofresearchCardiovascular medicine and haematology
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchcode3201
dc.subject.fieldofresearchcode3202
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsCardiac & Cardiovascular Systems
dc.subject.keywordsPeripheral Vascular Disease
dc.subject.keywordsCardiovascular System & Cardiology
dc.titlePacked Red Cell Age Associated With Adverse Cardiovascular Changes in an Ovine Model of Septic Shock Resuscitation
dc.typeConference output
dc.type.descriptionE3 - Conferences (Extract Paper)
dcterms.bibliographicCitationFanning, JP; Obonyo, NG; Tung, J-P; Byrne, L; Simonova, G; Diab, S; Dunster, K; Passmore, M; Boon, A-C; Hoe, SL; Engkilde-Pedersen, S; Esguerra-Lallen, A; Fauzi, HM; Anstey, C; et al., Packed Red Cell Age Associated With Adverse Cardiovascular Changes in an Ovine Model of Septic Shock Resuscitation, Circulation, 2018, 138
dc.date.updated2021-02-16T04:12:23Z
gro.hasfulltextNo Full Text
gro.griffith.authorAnstey, Christopher


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