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dc.contributor.authorUddin, MKM
dc.contributor.authorRahman, A
dc.contributor.authorAther, MF
dc.contributor.authorAhmed, T
dc.contributor.authorRahman, SMM
dc.contributor.authorAhmed, S
dc.contributor.authorBanu, S
dc.date.accessioned2021-02-17T01:42:36Z
dc.date.available2021-02-17T01:42:36Z
dc.date.issued2020
dc.identifier.issn1178-6973
dc.identifier.doi10.2147/IDR.S240408
dc.identifier.urihttp://hdl.handle.net/10072/402238
dc.description.abstractBackground: Rifampicin resistance (RR) is a key indicator of multidrug-resistant tuberculosis (MDR-TB) and 95% of the RR is associated with the mutation in the 81-bp rifampicin resistance determining region (RRDR) of the rpoB gene of Mycobacterium tuberculosis complex (MTBC). The Xpert MTB/RIF (Xpert) assay uses five overlapping molecular beacon probes (A-E) complementary to RRDR region that detect MTBC and mutations associated with RR. The objective of the study was to investigate the distribution and frequency of mutations detected by Xpert assay among Beijing and non-Beijing RR-TB isolates. Methods: A total of 205 randomly selected RR-TB specimens detected by Xpert assay were included in this study. A portion of specimens was further subjected to culture, MTBDRplus test and the positive culture isolates were genotyped by spoligotyping. Results: We found that the most frequent mutation occurred at probe E (S531L) binding region in both Beijing and non-Beijing isolates (61.9% and 66.9%, respectively). The Beijing family had higher mutation rates than non-Beijing (19.0% vs 12.4%) at probe B (D516V) while the non-Beijing family had higher mutations at probe D (H526D or H526Y) than the Beijing (13.2% vs 10.7%) family. Mutations at probes Aand C were less common in both Beijing and non-Beijing isolates. There was no significant difference (P=0.36) in the occurrence of mutations at different probes between Beijing and non-Beijing isolates. Conclusions: The study results revealed that the most frequent mutation occurs in the region of probe E and the least common mutations at probe A and C among both Beijing and non-Beijing RR-TB cases. This first insight into the probe mutation variation and frequencies among the RR-TB cases in Bangladesh forms the baseline information for further investigation.
dc.description.peerreviewedYes
dc.languageen
dc.publisherInforma UK Limited
dc.relation.ispartofpagefrom789
dc.relation.ispartofpageto797
dc.relation.ispartofjournalInfection and Drug Resistance
dc.relation.ispartofvolume13
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchPharmacology and pharmaceutical sciences
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode3214
dc.titleDistribution and frequency of rpob mutations detected by xpert MTB/RIF assay among beijing and non-beijing rifampicin resistant Mycobacterium tuberculosis isolates in Bangladesh
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationUddin, MKM; Rahman, A; Ather, MF; Ahmed, T; Rahman, SMM; Ahmed, S; Banu, S, Distribution and frequency of rpob mutations detected by xpert MTB/RIF assay among beijing and non-beijing rifampicin resistant Mycobacterium tuberculosis isolates in Bangladesh, Infection and Drug Resistance, 2020, 13, pp. 789-797
dcterms.licensehttp://creativecommons.org/licenses/by-nc/3.0/
dc.date.updated2021-02-17T01:13:19Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2020 Uddin et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms. php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php)
gro.hasfulltextFull Text
gro.griffith.authorRahman, Sabuktagin


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