Developing a marker toolbox for applications of olfactory glia for spinal cord injury therapies
Author(s)
Murtaza, M
Yao, R
Mohan, M
Barker, M
St John, J
Year published
2018
Metadata
Show full item recordAbstract
Background: Injury to the spinal cord can have devastating, life-changing consequences for patients and caregivers. Olfactory ensheathing cells (OECs) are specialized glia of the olfactory system with potent regenerative properties. Transplantation of OECs has resulted in significant functional recovery and is considered a promising treatment approach for spinal cord injury.
But consistent replication of outcomes is a challenge and a better understanding of the transplanted cell types is essential to improve therapeutic efficacy.
Methods: Olfactory mucosal biopsies were obtained by an ENT surgeon and were processed ...
View more >Background: Injury to the spinal cord can have devastating, life-changing consequences for patients and caregivers. Olfactory ensheathing cells (OECs) are specialized glia of the olfactory system with potent regenerative properties. Transplantation of OECs has resulted in significant functional recovery and is considered a promising treatment approach for spinal cord injury. But consistent replication of outcomes is a challenge and a better understanding of the transplanted cell types is essential to improve therapeutic efficacy. Methods: Olfactory mucosal biopsies were obtained by an ENT surgeon and were processed mechanically and enzymatically to generate a mixed culture of olfactory glial cells. The samples were assessed by immunocytochemistry using a panel of antibodies for OECs and fibroblasts. Results: By testing different conditions for culture we have optimized techniques for culture and in vitro expansion of human olfactory cells. Our data suggests that it is feasible to identify and define the different subsets of cells. Conclusion: Detailed characterization of the different cell types can eliminate some of the variability in functional outcomes. The results will facilitate optimization of the relative proportions of cells for maximal functional recovery in spinal cord injury repair.
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View more >Background: Injury to the spinal cord can have devastating, life-changing consequences for patients and caregivers. Olfactory ensheathing cells (OECs) are specialized glia of the olfactory system with potent regenerative properties. Transplantation of OECs has resulted in significant functional recovery and is considered a promising treatment approach for spinal cord injury. But consistent replication of outcomes is a challenge and a better understanding of the transplanted cell types is essential to improve therapeutic efficacy. Methods: Olfactory mucosal biopsies were obtained by an ENT surgeon and were processed mechanically and enzymatically to generate a mixed culture of olfactory glial cells. The samples were assessed by immunocytochemistry using a panel of antibodies for OECs and fibroblasts. Results: By testing different conditions for culture we have optimized techniques for culture and in vitro expansion of human olfactory cells. Our data suggests that it is feasible to identify and define the different subsets of cells. Conclusion: Detailed characterization of the different cell types can eliminate some of the variability in functional outcomes. The results will facilitate optimization of the relative proportions of cells for maximal functional recovery in spinal cord injury repair.
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Conference Title
Cytotherapy
Volume
20
Issue
5
Subject
Clinical sciences
Immunology
Medical biotechnology
Science & Technology
Life Sciences & Biomedicine
Cell & Tissue Engineering
Biotechnology & Applied Microbiology
Cell Biology