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  • Oxidative stress-induced insulin resistance in skeletal muscle cells is ameliorated by gamma-tocopherol treatment

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    Author(s)
    Singh, Indu
    Carey, Andrew L
    Watson, Nadine
    Febbraio, Mark A
    Hawley, John A
    Griffith University Author(s)
    Singh, Indu
    Year published
    2008
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    Abstract
    Background Oxidative stress-induced reactive oxygen species are associated with the clinical manifestation of insulin resistance. Evidence suggests that antioxidant treatment may reduce this incidence. Aim of the study This study determined whether glucose oxidase (GO)-induced insulin resistance in cultured skeletal muscle cells could be ameliorated by pre-treatment with gamma-tocopherol (GT). Methods Insulin sensitivity in L6 myotubes was assessed by 2-deoxy-d-[3H]-glucose uptake. The phosphorylation of distal insulin signaling proteins Akt and the Akt substrate AS160 were determined by western blot. Results ...
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    Background Oxidative stress-induced reactive oxygen species are associated with the clinical manifestation of insulin resistance. Evidence suggests that antioxidant treatment may reduce this incidence. Aim of the study This study determined whether glucose oxidase (GO)-induced insulin resistance in cultured skeletal muscle cells could be ameliorated by pre-treatment with gamma-tocopherol (GT). Methods Insulin sensitivity in L6 myotubes was assessed by 2-deoxy-d-[3H]-glucose uptake. The phosphorylation of distal insulin signaling proteins Akt and the Akt substrate AS160 were determined by western blot. Results One hour treatment with 100 mU/ml GO decreased insulin-stimulated glucose uptake (P < 0.001). Pre-treatment with GT either partially (100 卩 or completely (200 卩 restored insulin-stimulated glucose uptake in cells after GO-induced insulin resistance. GO-induced oxidative stress did not impair insulin stimulated phosphorylation of Akt or AS160, but 200 占GT increased insulin-stimulated phosphorylation of these key signaling proteins (P < 0.05). Conclusions High-dose (200 卩 GT treatment ameliorated oxidative stress-induced insulin resistance in cultured rat L6 skeletal muscle cells.
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    Journal Title
    European Journal of Nutrition
    Volume
    47
    Issue
    7
    DOI
    https://doi.org/10.1007/s00394-008-0739-2
    Copyright Statement
    © 2008 Springer Berlin / Heidelberg. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. The original publication is available at www.springerlink.com
    Subject
    Cardiovascular medicine and haematology not elsewhere classified
    Nutrition and dietetics
    Publication URI
    http://hdl.handle.net/10072/40252
    Collection
    • Journal articles

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