Show simple item record

dc.contributor.authorUmer, Muhammad
dc.contributor.authorQureshi, Sohail Asif
dc.contributor.authorHashmi, Zahid Yasin
dc.contributor.authorRaza, Asif
dc.contributor.authorAhmad, Janbaz
dc.contributor.authorRahman, Moazur
dc.contributor.authorIqbal, Mazhar
dc.date.accessioned2021-02-23T04:57:38Z
dc.date.available2021-02-23T04:57:38Z
dc.date.issued2014
dc.identifier.issn1743-422X
dc.identifier.doi10.1186/1743-422X-11-117
dc.identifier.urihttp://hdl.handle.net/10072/402523
dc.description.abstractBackground: Aberrant DNA methylation profiles are a characteristic feature of almost all types of cancers including hepatocellular carcinoma (HCC) and play an important role in carcinogenesis. In spite of the accumulating evidence that suggests appearance of such aberrations at precancerous stages, very little effort has been invested to investigate such possible methylation events in patients at risk of developing HCC i.e. those suffering from chronic hepatitis C virus (HCV) infection and liver cirrhosis (LC). We reasoned that such an analysis could lead to the identification of novel predictive biomarkers as well as potential drug targets. Methods. Promoter methylation status of two Wnt inhibitors SFRP2 and DKK1 was quantitatively analyzed by bisulfite pyrosequencing in a series of liver biopsy samples. These biopsies were collected from HCV-infected individuals suffering from chronic hepatitis (CH; n = 15), liver cirrhosis (LC; n = 13) and hepatocellular carcinoma (HCC; n = 41). DNA isolated from infection free normal livers (N; n =10) was used as control. Results: Our analysis revealed that both of the genomic loci were significantly hypermethylated in CH patients' livers as compared to normal controls (p = 0.0136 & 0.0084 for SFRP2 and DKK1, respectively; Mann-Whitney U test). DNA methylation levels for both loci were also significantly higher in all the diseased cohorts as compared to normal controls (p < 0.0001 and = 0.0011 for SFRP2 and DKK1, respectively; Kruskal-Wallis test). However, a comparison between three disease cohorts (CH, LC & HCC) revealed no significant difference in levels of DNA methylation at DKK1 promoter. In contrast, a progressive increase in DNA methylation levels was observed at the SFRP2 promoter (i.e. N < CH & LC < HCC). Conclusion: This study demonstrated that in HCV infected liver tissues hypermethylation at promoter regions of key cancer related genes SFRP2 and DKK1, appears early at CH and LC stages, long before the appearance of HCC.
dc.description.peerreviewedYesen_US
dc.languageEnglishen_US
dc.publisherBMC
dc.relation.ispartofpagefrom117
dc.relation.ispartofissue1
dc.relation.ispartofjournalVirology Journal
dc.relation.ispartofvolume11
dc.subject.fieldofresearchMicrobiologyen_US
dc.subject.fieldofresearchMedical Microbiologyen_US
dc.subject.fieldofresearchcode0605en_US
dc.subject.fieldofresearchcode1108en_US
dc.subject.keywordsScience & Technologyen_US
dc.subject.keywordsLife Sciences & Biomedicineen_US
dc.subject.keywordsVirologyen_US
dc.subject.keywordsHypermethylationen_US
dc.subject.keywordsWnt pathwayen_US
dc.titlePromoter hypermethylation of Wnt pathway inhibitors in hepatitis C virus - induced multistep hepatocarcinogenesis
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationUmer, M; Qureshi, SA; Hashmi, ZY; Raza, A; Ahmad, J; Rahman, M; Iqbal, M, Promoter hypermethylation of Wnt pathway inhibitors in hepatitis C virus - induced multistep hepatocarcinogenesis, Virology Journal, 2014, 11 (1), pp. 117
dcterms.dateAccepted2014-06-12
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/en_US
dc.date.updated2021-02-23T04:55:23Z
dc.description.versionVersion of Record (VoR)en_US
gro.rights.copyright© 2014 Umer et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en_US
gro.hasfulltextFull Text
gro.griffith.authorUmer, Muhammad


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record