dc.contributor.author | Khatun, Farjana | |
dc.contributor.author | Dai, Charles C | |
dc.contributor.author | Rivera-Hernandez, Tania | |
dc.contributor.author | Hussein, Waleed M | |
dc.contributor.author | Khalil, Zeinab G | |
dc.contributor.author | Capon, Robert J | |
dc.contributor.author | Toth, Istvan | |
dc.contributor.author | Stephenson, Rachel J | |
dc.date.accessioned | 2021-03-02T22:21:42Z | |
dc.date.available | 2021-03-02T22:21:42Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 2373-8227 | |
dc.identifier.doi | 10.1021/acsinfecdis.0c00722 | |
dc.identifier.uri | http://hdl.handle.net/10072/402736 | |
dc.description.abstract | Identifying the immunogenic moieties and their precise structure of carbohydrates plays an important role for developing effective carbohydrate-based subunit vaccines. This study assessed the structure–immunogenicity relationship of carbohydrate moieties of a single repeating unit of group A carbohydrate (GAC) present on the cell wall of group A Streptococcus (GAS) using a rationally designed self-adjuvanted lipid-core peptide, instead of a carrier protein. Immunological evaluation of fully synthetic glyco-lipopeptides (particle size: 300–500 nm) revealed that construct consisting of higher rhamnose moieties (trirhamnosyl-lipopeptide) was able to induce enhanced immunogenic activity in mice, and GlcNAc moiety was not found to be an essential component of immunogenic GAC mimicked epitope. Trirhamnosyl-lipopeptide also showed 75–97% opsonic activity against four different clinical isolates of GAS and was comparable to a subunit peptide vaccine (J8-lipopeptide) which illustrated 65–96% opsonic activity. | |
dc.description.peerreviewed | Yes | |
dc.language | en | |
dc.publisher | American Chemical Society (ACS) | |
dc.relation.ispartofpagefrom | 390 | |
dc.relation.ispartofpageto | 405 | |
dc.relation.ispartofissue | 2 | |
dc.relation.ispartofjournal | ACS Infectious Diseases | |
dc.relation.ispartofvolume | 7 | |
dc.subject.fieldofresearch | Medical microbiology | |
dc.subject.fieldofresearchcode | 3207 | |
dc.title | Immunogenicity Assessment of Cell Wall Carbohydrates of Group A Streptococcus via Self-Adjuvanted Glyco-lipopeptides | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dcterms.bibliographicCitation | Khatun, F; Dai, CC; Rivera-Hernandez, T; Hussein, WM; Khalil, ZG; Capon, RJ; Toth, I; Stephenson, RJ, Immunogenicity Assessment of Cell Wall Carbohydrates of Group A Streptococcus via Self-Adjuvanted Glyco-lipopeptides, ACS Infectious Diseases, 2021, 7 (2), pp. 390-405 | |
dc.date.updated | 2021-03-01T22:03:20Z | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Dai, Charles C. | |